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Anti lamin a c sc 20681

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Anti-Lamin A/C (sc-20681) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect lamin A/C proteins in various sample types.

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Lab products found in correlation

Ab9194, by Abcam (2 mentions) Anti gapdh sc 25778, by Santa Cruz Biotechnology (2 mentions) Anti ubiquitin, by Merck Group (2 mentions) Anti chrebp, by Novus Biologicals (2 mentions) Anti β tubulin t5201, by Merck Group (2 mentions) Sc 101006, by Santa Cruz Biotechnology (2 mentions) Anti cbp sc 33000, by Santa Cruz Biotechnology (2 mentions) Nb400 135, by Merck Group (2 mentions) Aqp 005, by Abcam (1 mentions) Fluorchem 8900 imaging system, by Bio-Techne (1 mentions) Anti actb, by Abcam (1 mentions) Anti actin sc 1616, by Santa Cruz Biotechnology (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions)

Spelling variants of this product

Lamin A/C (177 citations) Anti-Lamin A/C (73 citations) Lamin A (27 citations) Anti-Lamin A (18 citations) Sc-20681 (12 citations) Lamin A/C (sc-20681 (5 citations)

4 protocols using anti lamin a c sc 20681

1

Isolation and Analysis of Protein Ubiquitination

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Liver tissues or cell pellets were lysed in hypotonic buffer (5 mM Tris-HCl, 1 mM MgCl2, 3 mM CaCl2, 8% Sucrose). After centrifuge, pellets were washed once with hypotonic buffer and re-suspended in RIPA buffer (5 mM Tris-HCl, 150 mM NaCl, 0.1% SDS, 0.05% deoxycholic acid, 10% Glycerol) prior to sonication for 5 s. Nuclear fractions were then collected after centrifugation at 13,000 rpm × 10 min. FLAG-M2 beads or streptavidin beads were added into nuclear fractions to capture FLAG-CRY1 or CBP-CRY1. The protocol for detecting ubiquitination was reported [20 (link)] with minor modifications. anti-ChREBP was used to pull down poly-ubiquitinated ChREBPα conjugates. Western blot analysis was performed using the following primary antibodies: anti-DDB1 (Abcam ab9194), anti-CRY1 (sc-101006), anti-GAPDH (sc-25778), anti-Lamin A/C (sc-20681), anti-CBP (sc-33000) (Santa Cruz biotechnology), anti-ChREBP (Novus NB400–135), anti-ubiquitin (Sigma U5379), and anti-β-tubulin (T5201) (Sigma-Aldrich).
Tong X., Zhang D., Shabandri O., Oh J., Jin E., Stamper K., Yang M., Zhao Z, & Yin L. (2020). DDB1 E3 ligase controls dietary fructose-induced ChREBPα stabilization and liver steatosis via CRY1. Metabolism: clinical and experimental, 107, 154222.
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2

Protein Analysis via Western Blot

Cited 1 time
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Western blots were performed as previously described [8 (link)]. Primary antibodies (all rabbit) were anti-AQP5 (Alomone Labs AQP-005), anti-CAV1 (Abcam ab2910), anti-pro-SFTPC (Millipore AB3786), anti-ACTB (Abcam AB8226), anti-PDPN (Developmental Studies Hybridoma Bank #8.1.1), and anti-LAMIN A/C (sc-20,681, Santa Cruz Biotechnology). Blots were analyzed by chemiluminescence and visualized by West Fempto Super Sensitivity Kit (Thermo Scientific) with a FluorChem 8900 Imaging System (Alpha Innotech).
Zhou B., Stueve T.R., Mihalakakos E.A., Miao L., Mullen D., Wang Y., Liu Y., Luo J., Tran E., Siegmund K.D., Lynch S.K., Ryan A.L., Offringa I.A., Borok Z, & Marconett C.N. (2021). Comprehensive epigenomic profiling of human alveolar epithelial differentiation identifies key epigenetic states and transcription factor co-regulatory networks for maintenance of distal lung identity. BMC Genomics, 22, 906.
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3

Isolation and Analysis of Protein Ubiquitination

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Liver tissues or cell pellets were lysed in hypotonic buffer (5 mM Tris-HCl, 1 mM MgCl2, 3 mM CaCl2, 8% Sucrose). After centrifuge, pellets were washed once with hypotonic buffer and re-suspended in RIPA buffer (5 mM Tris-HCl, 150 mM NaCl, 0.1% SDS, 0.05% deoxycholic acid, 10% Glycerol) prior to sonication for 5 s. Nuclear fractions were then collected after centrifugation at 13,000 rpm × 10 min. FLAG-M2 beads or streptavidin beads were added into nuclear fractions to capture FLAG-CRY1 or CBP-CRY1. The protocol for detecting ubiquitination was reported [20 (link)] with minor modifications. anti-ChREBP was used to pull down poly-ubiquitinated ChREBPα conjugates. Western blot analysis was performed using the following primary antibodies: anti-DDB1 (Abcam ab9194), anti-CRY1 (sc-101006), anti-GAPDH (sc-25778), anti-Lamin A/C (sc-20681), anti-CBP (sc-33000) (Santa Cruz biotechnology), anti-ChREBP (Novus NB400–135), anti-ubiquitin (Sigma U5379), and anti-β-tubulin (T5201) (Sigma-Aldrich).
Tong X., Zhang D., Shabandri O., Oh J., Jin E., Stamper K., Yang M., Zhao Z, & Yin L. (2020). DDB1 E3 ligase controls dietary fructose-induced ChREBPα stabilization and liver steatosis via CRY1. Metabolism: clinical and experimental, 107, 154222.
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4

Western Blot Analysis of Lamin Proteins

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Nuclei were homogenized in 50 mM tris (pH 7.4), 150 mM NaCl, 1% NP-40, 50 mM NaF, 1 mM DTT, and complete protease inhibitor cocktail (2 mg/ml; Roche). The whole amount of protein from homogenates was quantified using a BCA Protein Assay Kit (Pierce), and 15 g from each sample was loaded in SDS-polyacrylamide gels. After electrophoresis at 120 mV, gels were electrotransferred onto polyvinylidene fluoride membranes, blocked with 3% nonfat dry milk, and incubated overnight at 4°C with 1:2000 anti-Lamin-A/C (sc-20681, Santa Cruz Biotechnology), 1:1000 anti-Lamin-B1 (sc-374015, Santa Cruz Biotechnology), and 1:10,000 anti-actin (sc-1616, Santa Cruz Biotechnology) in tris-buffered saline with 0.05% Tween 20. Finally, blots were incubated with 1:10,000 goat anti-rabbit horseradish peroxidase (HRP; sc-2004, Santa Cruz Biotechnology) or 1:10,000 anti-mouse HRP (sc-2005, Santa Cruz Biotechnology) in 1.5% nonfat milk. Proteins were detected by chemiluminescence in a ChemiDoc camera (UVP), and the intensity of each band was quantified in digital images using the ImageJ software (NIH).
López-Alonso I., Blázquez-Prieto J., Amado-Rodríguez L., González-López A., Astudillo A., Sánchez M., Huidobro C., López-Martínez C., López-Martínez C., Dos Santos C.C, & Albaiceta G.M. (2018). Preventing loss of mechanosensation by the nuclear membranes of alveolar cells reduces lung injury in mice during mechanical ventilation. Science translational medicine, 10(456).
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