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Varian inova 500 mhz nmr spectrometer

Manufactured by Agilent Technologies

The Varian INOVA 500-MHz NMR spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument that operates at a frequency of 500 MHz. It is designed for the analysis and characterization of chemical compounds and materials. The core function of this spectrometer is to generate a strong magnetic field and detect the radio frequency signals emitted by the nuclei of atoms within a sample, providing detailed information about the molecular structure and composition of the analyzed substance.

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2 protocols using varian inova 500 mhz nmr spectrometer

1

NMR Characterization of Synthesized Compounds

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The purity and identity of the synthesized compounds were determined by 1H NMR. All the acquisitions were carried out in D2O on a Varian INOVA 500‐MHz NMR spectrometer (Agilent Technologies).
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2

NMR Characterization of Lyophilized Products

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Lyophilized products were dissolved to 3−10 mM in 50 mM potassium phosphate buffer (pD 7.9) in a total volume of 600 μL and transferred to a 5 mm high-precision NMR sample tube. They were analyzed using a Varian INOVA 500-MHz NMR spectrometer (Agilent Technologies). The VNMRJ 2.2D software was used for measurements. 1H-NMR spectra (499.98 MHz) were measured on a 5 mm indirect detection PFG probe. Standard pre-saturation sequence was used: relaxation delay 2 s; 908 proton pulse; 2.048 s acquisition time; spectral width 8 kHz; number of points 32,000 13C NMR spectra (125.71 MHz). Standard pre-saturation sequence was used: relaxation delay 2 s; 908 proton pulse; 2.048 s acquisition time; spectral width 8 kHz; number of points 32 k. MNova 12.0.3 (Mestrelab Research S.L. Bajo, Santiago de Compostela, Spain) was used for data processing and analysis (Supplementary Figs. 1029). Recorded spectra were in good agreement with published data75 (link).
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