1 ethyl 3 dimethylaminopropyl carbodiimide hydrochloride edc

Fresh cocoons of Bombyx mori silkworm were obtained from College of Biotechnology, Southwest University. Sodium carbonate anhydrous (Na₂CO₃), calcium chloride anhydrous (CaCl₂), and N,N-dimethylformamide (DMF) were purchased from Kelong Chemical Reagent Factory (Chengdu, China) and at AR grade. Anhydrous ethanol (CH₃CH₂OH, AR grade) was purchased from Chongqing Chuandong Chemical Group Co., Ltd. (Chongqing, China). 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Solarbio (Beijing, China). Anhydrous dimethyl sulfoxide (DMSO), dibutyltin dilaurate and hydroquinone were purchased from Aladdin Industrial Co., Ltd. (Shanghai, China). 2-Isocyanatoethyl methacrylate (MOI) was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). 1H,1H,2H,2H-perfluorodecanethiol (PFDT) and 1H,1H,2H,2H-perfluoro-1-decanol (FTOH) were purchased from Sigma-Aldrich (Saint-Louis, USA). Ultrapure water (resistance > 18 MΩ cm⁻¹) was used in all experiments.

Fresh cocoons of Bombyxmori silkworm were obtained from College of Biotechnology, Southwest University. Both sodium carbonate anhydrous (Na₂CO₃) and calcium chloride anhydrous (CaCl₂) were purchased from Kelong chemical reagent factory (Chengdu, China) and at AR grade. Anhydrous ethanol (C₂H₆O, AR grade) was purchased from Chongqing Chuandong chemical group Co. (Chongqing, China). 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Solarbio (Beijing, China). DMEM (high glucose) and PBS (1×) were purchased from Hyclone (Utah, United States). Ultrapure water (resistance > 18 MΩ cm⁻¹) was used in all experiments.