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Opsys mrtm 15

Manufactured by Dynex
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Sourced in United Kingdom

The Opsys MRTM 15 is a laboratory equipment designed for microscopy and imaging applications. It is a multi-resolution transmitted light microscope that provides high-quality imaging at various magnification levels.

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Opsys MR (40 citations) Opsys MRTM (7 citations)

2 protocols using opsys mrtm 15

1

Metabolic Activity Assay for Cell Proliferation

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Metabolic activity was used as a surrogate for cell proliferation, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [9 (link)]. Cells were seeded into 96-well plates. After overnight attachment, TAM (0.1-100 nM), E2 (0.01–1 µM), PGE2 (1 µM), butaprost or PGE1-OH (both 0.1–1 µM) or ethanol vehicle was added. At 96 h post-treatment, cells were incubated with 1 mM MTT for 4 h. The purple formazan product was solubilised in propan-1-ol and read at 570 nm using an Opsys MRTM 15 (Dynex Technologies, UK). Chemicals were purchased from Sigma-Aldrich (UK) or Cayman Chemical (USA).
Volpato M., Cummings M., Shaaban A.M., Abderrahman B., Hull M.A., Maximov P.Y., Broom B.M., Hoppe R., Fan P., Brauch H., Jordan V.C, & Speirs V. (2020). Downregulation of 15-hydroxyprostaglandin dehydrogenase during acquired tamoxifen resistance and association with poor prognosis in ERα-positive breast cancer. Exploration of Targeted Anti-tumor Therapy, 1(5), 355-371.
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2

Evaluation of Metabolic Activity in Cell Proliferation

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Metabolic activity was used as a surrogate for cell proliferation, using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay [9 (link)]. Cells were seeded into 96-well plates. After overnight attachment, TAM (0.1-100 nM), E2 (0.01-1 µM), PGE2 (1 µM), butaprost or PGE1-OH (both 0.1-1 µM) or ethanol vehicle was added. At 96 h post-treatment, cells were incubated with 1 mM MTT for 4 h. The purple formazan product was solubilised in propan-1-ol and read at 570 nm using an Opsys MRTM 15 (Dynex Technologies, UK). Chemicals were purchased from Sigma-Aldrich (UK) or Cayman Chemical (USA).
Volpato M., Cummings M., Shaaban A.M., Abderrahman B., Hull M.A., Maximov P.Y., Broom B.M., Hoppe R., Fan P., Brauch H., Jordan V.C, & Speirs V. (2020). Downregulation of 15-hydroxyprostaglandin dehydrogenase during acquired tamoxifen resistance and association with poor prognosis in ERα-positive breast cancer. Exploration of Targeted Anti-tumor Therapy, 1(5), 355-371.
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