Thirty thousand KSL cells were sorted into 10% trichloroacetic acid, pelleted at 14,000 rpm for 10 min at 4°C, and washed twice with acetone. Dried pellets were resuspended in solubilization buffer (9 mol/L urea, 2% Triton X-100, and 1% dithiothreitol [DTT]), and incubated with loading dye at 70°C for 10 min. Samples were run on 4%–12% Bis-Tris Plus Gels (Invitrogen, Carlsbad, CA) and transferred to poly-vinyl difluoride (PVDF) membranes. Membranes were probed for phospho-STAT1 (No. 9167, Cell Signaling, Danvers), STAT1 (No. 9172, Cell Signaling), pErk1/2 (No. 4370, Cell Signaling), Erk1/2 (No. 4696, Cell Signaling), and rabbit anti-mouse HRP-conjugated secondary antibody (No. 7074, Cell Signaling). Proteins were detected using Pierce ECL Western Blotting Substrate (ThermoFisher, Kalamazoo, MI) on chemiluminescence film.
Rabbit anti mouse hrp conjugated secondary antibody
Manufactured by Cell Signaling Technology
Rabbit anti-mouse HRP-conjugated secondary antibody is a laboratory reagent used to detect and quantify mouse primary antibodies in various immunoassays. It is a polyclonal antibody raised in rabbits against mouse immunoglobulins and is conjugated with horseradish peroxidase (HRP), an enzyme that catalyzes color-producing reactions for signal detection.
Automatically generated - may contain errors
Lab products found in correlation
Erk1 2, by Cell Signaling Technology (2 mentions)
P erk1 2, by Cell Signaling Technology (2 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Bis tris plus gel, by Thermo Fisher Scientific (2 mentions)
Stat1, by Cell Signaling Technology (2 mentions)
Phospho stat1, by Cell Signaling Technology (2 mentions)
2 protocols using rabbit anti mouse hrp conjugated secondary antibody
1
Protein Extraction and Western Blot Analysis
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thirty thousand KSL cells were sorted into 10% trichloroacetic acid, pelleted at 14,000 rpm for 10 min at 4°C, and washed twice with acetone. Dried pellets were resuspended in solubilization buffer (9 mol/L urea, 2% Triton X-100, and 1% dithiothreitol [DTT]), and incubated with loading dye at 70°C for 10 min. Samples were run on 4%–12% Bis-Tris Plus Gels (Invitrogen, Carlsbad, CA) and transferred to poly-vinyl difluoride (PVDF) membranes. Membranes were probed for phospho-STAT1 (No. 9167, Cell Signaling, Danvers), STAT1 (No. 9172, Cell Signaling), pErk1/2 (No. 4370, Cell Signaling), Erk1/2 (No. 4696, Cell Signaling), and rabbit anti-mouse HRP-conjugated secondary antibody (No. 7074, Cell Signaling). Proteins were detected using Pierce ECL Western Blotting Substrate (ThermoFisher, Kalamazoo, MI) on chemiluminescence film.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!