Log In Sign up
The largest database of trusted experimental protocols

Dicyanocobinamide cbi

Manufactured by Merck Group
Visit Supplier

Dicyanocobinamide (Cbi) is a laboratory reagent used in the analysis and characterization of various biological samples. It is a cobalamin-related compound that serves as a precursor or intermediate in the biosynthesis of vitamin B12 (cobalamin). Cbi can be utilized in spectroscopic and chromatographic techniques to study and quantify cobalamin and related molecules in biological matrices.

Automatically generated - may contain errors

Lab products found in correlation

Vitamin b12, by Merck Group (2 mentions) Lambda 35 uv vis spectrometer, by PerkinElmer (2 mentions) Yeast extract, by BD (2 mentions) Peptone, by BD (2 mentions) Vinyl chloride, by Merck Group (2 mentions) Ethene, by Merck Group (2 mentions) Purine, by Merck Group (2 mentions) 15nh4cl, by Cambridge Isotopes (2 mentions) 5 6 dimethylbenzimidazole dmb, by Merck Group (2 mentions) 5 methylbenzimidazole 5 mebza, by Merck Group (2 mentions) 5 methoxybenzimidazole 5 omebza, by Merck Group (2 mentions) Benzimidazole bza, by Merck Group (2 mentions)

Spelling variants of this product

Dicyanocobinamide (6 citations)

2 protocols using dicyanocobinamide cbi

1

Biosynthesis and Purification of Cobamides

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vitamin B12 (≥98%), dicyanocobinamide (Cbi, ≥93%), 5,6-dimethylbenzimidazole (DMB) (99%), 5-methylbenzimidazole (5-MeBza) (98%), 5-methoxybenzimidazole (5-OMeBza) (97%), benzimidazole (Bza) (98%), purine (98%) were purchased from Sigma-Aldrich. 15NH4Cl (99%) was obtained from Cambridge Isotope Laboratories. Yeast extract and peptone were purchased from Becton, Dickinson and Company. Bza-Cba, 5-MeBza-Cba and 5-OMeBza-Cba were prepared via guided cobamide biosynthesis 10 (link). Factor III (5-OHBza-Cba), norpseudo vitamin B12 and phenolic cobamides (i.e., Phenol-Cba and p-Cresol-Cba) were extracted and purified from Methanosarcina barkeri strain Fusaro (DSM 804), Sulfurospirillum multivorans (DSM 12446) and Sporomusa sp. strain KB-1 (16S rRNA gene GenBank accession no. AY780559.1) cells, respectively. Concentrations of purified cobamides were determined at 361 nm with a Lambda 35 UV-Vis spectrometer (PerkinElmer) using a molar extinction coefficient of 28,060 mole−1 cm−1 12 (link). Ethene (≥99.9%) and vinyl chloride (≥99.5%) were purchased from Sigma-Aldrich. All other chemicals used were reagent grade or higher.
Yan J., Bi M., Bourdon A.K., Farmer A.T., Wang P.H., Molenda O., Quaile A., Jiang N., Yang Y., Yin Y., Şimşir B., Campagna S.R., Edwards E.A, & Löffler F.E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature chemical biology, 14(1), 8-14.
+ Open protocol
+ Expand
2

Biosynthesis and Purification of Cobamides

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vitamin B12 (≥98%), dicyanocobinamide (Cbi, ≥93%), 5,6-dimethylbenzimidazole (DMB) (99%), 5-methylbenzimidazole (5-MeBza) (98%), 5-methoxybenzimidazole (5-OMeBza) (97%), benzimidazole (Bza) (98%), purine (98%) were purchased from Sigma-Aldrich. 15NH4Cl (99%) was obtained from Cambridge Isotope Laboratories. Yeast extract and peptone were purchased from Becton, Dickinson and Company. Bza-Cba, 5-MeBza-Cba and 5-OMeBza-Cba were prepared via guided cobamide biosynthesis 10 (link). Factor III (5-OHBza-Cba), norpseudo vitamin B12 and phenolic cobamides (i.e., Phenol-Cba and p-Cresol-Cba) were extracted and purified from Methanosarcina barkeri strain Fusaro (DSM 804), Sulfurospirillum multivorans (DSM 12446) and Sporomusa sp. strain KB-1 (16S rRNA gene GenBank accession no. AY780559.1) cells, respectively. Concentrations of purified cobamides were determined at 361 nm with a Lambda 35 UV-Vis spectrometer (PerkinElmer) using a molar extinction coefficient of 28,060 mole−1 cm−1 12 (link). Ethene (≥99.9%) and vinyl chloride (≥99.5%) were purchased from Sigma-Aldrich. All other chemicals used were reagent grade or higher.
Yan J., Bi M., Bourdon A.K., Farmer A.T., Wang P.H., Molenda O., Quaile A., Jiang N., Yang Y., Yin Y., Şimşir B., Campagna S.R., Edwards E.A, & Löffler F.E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature chemical biology, 14(1), 8-14.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!