Human prostate cancer DU145 and PC-3 cell lines were from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco’s Modified Eagle’s medium/1640 supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin, at 37°C, in humidified air containing 5% CO2.
Salen-Mn has been synthesized from Xi’an Jiaotong University [8 (link)]. Salen-Mn was made into a fine suspension by dissolving the compound in DMSO, which was diluted to desired concentrations in the medium immediately before each experiment. 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenylSalen-Mnrazolium bromide (MTT) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 3-methyadenine (3-MA, 189490) was purchased from Merck Millipore (Darmstadt, Germany). Antibodies against cleaved caspase-3, poly (ADP-ribose) polymerase (PARP), Bcl-2, BAX, LC3-I/II, p-AMPK, p-ACC, p-mTOR and peroxidase-conjugated secondary antibodies were from Cell Signaling Technology (Beverly, MA, USA). Antibody against glyceraldehyde-3-phosphate dehydrogenase was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The enhanced chemiluminescence (ECL) detection system was obtained from Amersham Life Science, Inc. (Arlington Heights, IL, USA).
Salen-Mn has been synthesized from Xi’an Jiaotong University [8 (link)]. Salen-Mn was made into a fine suspension by dissolving the compound in DMSO, which was diluted to desired concentrations in the medium immediately before each experiment. 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenylSalen-Mnrazolium bromide (MTT) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 3-methyadenine (3-MA, 189490) was purchased from Merck Millipore (Darmstadt, Germany). Antibodies against cleaved caspase-3, poly (ADP-ribose) polymerase (PARP), Bcl-2, BAX, LC3-I/II, p-AMPK, p-ACC, p-mTOR and peroxidase-conjugated secondary antibodies were from Cell Signaling Technology (Beverly, MA, USA). Antibody against glyceraldehyde-3-phosphate dehydrogenase was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The enhanced chemiluminescence (ECL) detection system was obtained from Amersham Life Science, Inc. (Arlington Heights, IL, USA).