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2 protocols using orange 2

1

Synthesis and Characterization of Polymeric Microspheres

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2-Methoxyethyl acrylate (MEA, purity 98%),
styrene (St, 99%), butyl acrylate (BA, 99%), potassium peroxodisulfate
(KPS, 95%), sodium dodecyl sulfate (SDS, 95%), disodium hydrogenphosphate
(99%), eosin Y (EoY, 95%), phloxine B (PhB, 98%), erythrosine (Ery,
95%), rose bengal (RB, 95%), orange II (OrII, 98%), tartrazine (Ttz,
98%), and ethanol (EtOH, 99.5%) were purchased from Wako Pure Chemical
Industries and used as received. 2-(2-Ethoxyethoxy) ethyl acrylate
(ET2A, 98%), 4-methoxystyrene (MSt, 98%), N-isopropylacrylamide
(NIPAm, 98%), and N,N′-methylenebis(acrylamide)
(BIS, 97%) were purchased from Tokyo Chemical Industry and used as
received. 2-(2-Methoxyetoxy) ethyl acrylate (ME2A, 95%) was purchased
from Monomer-Polymer and Dajac Labs, Inc. 2-[2-(2-Methoxyethoxy)ethoxy]ethyl
acrylate (Me3A, 98%) was kindly donated by Kyoeisha Chemical Co.,
Ltd. The cross-linker, ethylene glycol dimethacrylate (EGDMA, 98%),
was purchased from Sigma-Aldrich and used as received. Water used
for microsphere preparations was distilled and then ion-exchanged
(EYELA, SA-2100E1).
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2

Degradation of Dyes by B. multivorans CCA53

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CCA53 was previously deposited in the HUT culture collection under strain number HUT-8135. Bordeaux S, bromothymol blue, congo red, cresol red, curcumin, ethyl red, fluorescein, methyl orange, methyl red, orange II, rhodamine B, thymol blue, and trypan blue were purchased from Fujifilm Wako Chemicals (Osaka, Japan) or Tokyo Chemical Industry (Tokyo, Japan). To confirm dyedegradation capacity, B. multivorans CCA53 was aerobically grown on LB plates (pH 7.0) containing 100 µM each dye for 3 days at 30°C.
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