After stimulation of the CD4+ T cells was completed, the supernatant was collected to detect the concentration of IFN-γ and IL-4 by ELISA according to the manufacturer's protocol (mouse IL-4 IFN-γ kit, MultiSciences, China; mouse IL-4 ELISA kit, MultiSciences, China). A standard plate reader was used to read absorbance at 450 nm. The establishment of the standard curve was based on the optical density (OD) measurements. The required cytokine concentrations were extrapolated from this standard curve.
Mouse il 4 elisa kit
Manufactured by MultiSciences Biotech
Sourced in China
The Mouse IL-4 ELISA kit is a laboratory tool used to quantify the levels of interleukin-4 (IL-4) in mouse biological samples. IL-4 is a cytokine that plays a crucial role in immune system regulation. This kit employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of IL-4 present in the sample.
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Lab products found in correlation
2 protocols using mouse il 4 elisa kit
1
Quantification of T Cell Cytokines
2
Cytokine Quantification in Biological Samples
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IL-4, IL-5, IL-13, and TL1A levels in plasma or cell culture supernatant were measured using commercial ELISA kits. The ELISA kits used were as follows: Mouse IL-4 ELISA Kit (70-EK204/2-96; MultiSciences, Hangzhou, China), Mouse IL-5 ELISA Kit (70-EK105-96; MultiSciences), Mouse IL-13 ELISA Kit (70-EK204/2-96, 70-EK213/2-96; MultiSciences), Mouse IL-4 ELISA Kit, Mouse TNFSF15 ELISA Kit (ZC-55212; ZCI BIO, Shanghai, China), and Human TNFSF15 ELISA Kit (ZC-35762; ZCI BIO).
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