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Hrp linked anti rabbit secondary antibodies

Manufactured by Bioworld Technology
Sourced in United States

HRP-linked anti-rabbit secondary antibodies are a type of lab equipment used to detect and measure the presence of rabbit primary antibodies in various immunoassay techniques, such as Western blotting and ELISA. These secondary antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a colorimetric or chemiluminescent reaction, allowing for the visualization and quantification of the target rabbit antibodies.

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2 protocols using hrp linked anti rabbit secondary antibodies

1

As2O3 Protein Signaling Pathway

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As2O3 was purchased from YIDA (Harbin, China). A stock solution of As2O3 was diluted into indicated concentrations when needed. The following primary antibodies were used: FOXO3a and CD34 were purchased from Abcam (Cambridge, MA, USA); AKT, p-AKT, P38, p-P38, ERK, and p-ERK were purchased from Cell Signaling Technology (Boston, MA, USA). VEGF and MMP9 were purchased from Proteintech (Wuhan, China). GAPDH and HRP-linked anti-rabbit secondary antibodies were purchased from Bioworld Technology (Louis Park, MN, USA).
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2

Cellular Signaling Pathway Analysis

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EGF was purchased from R&D Systems (Minneapolis, MN, USA). Phospho-ERK1/2, phospho-AKT (Thr308), phospho-AKT (Ser473), AKT, β-catenin, ERK1/2, and Histone3 antibodies were purchased from Cell Signaling Technology (Boston, MA, USA). MMP9 antibody was purchased from Bimake (Shanghai, China). GAPDH antibody and HRP-linked anti-rabbit secondary antibodies were purchased from Bioworld Technology (Louis Park, MN, USA). WNT7A antibody was purchased from Abcam (Cambridge, MA, USA). MEK inhibitor U0126 was purchased from Promega (Madison, WI, USA), and PI3K/AKT inhibitor LY294402 was purchased from Sigma (St. Louis, MO, USA).
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