Leo supra 55 vp

SEM images of the as‐deposited thin Ni(OH)₂ films were obtained using a LEO Supra 55 VP (Zeiss) scanning electron microscope at an accelerating voltage of 5 kV.

REF52 cells were cultured on FN-coated mica for 10, 60, or 240 min or overnight and fixed with 1% glutaraldehyde/4% PFA in 0.1 M cacodylate buffer (pH 7.2) at room temperature for 1 h. Afterward, samples were progressively dehydrated in a 30, 50, 70, 90, and 100% ethanol series for 5 min each and critical point-dried (EM cpd300; Leica, Wetzlar, Germany). The samples were sputter-coated with 5-nm platinum and imaged with a LeoSupra55VP scanning electron microscope (Zeiss), using an aperture size of 20 μm and an accelerating voltage of 7.0 kV.

All the chemicals were purchased from Sigma-Aldrich Co and were used as received without further purification. A PEVA copolymer with a VA content of 12 wt% was used as the composite matrix. Meting points were measured using a SMP30 (BIBBY) melting point meter. Fourier-transform infrared (FTIR) spectra were recorded using an Hyperion 2000 (Bruker Optics) FTIR system. A JEOL instrument operated at 400 and 100 MHz was used to obtain ¹H nuclear magnetic resonance (NMR) and ¹³C NMR spectra. The chemical shifts were measured in parts per million using a deuterated solvent as the internal reference. High-resolution mass spectroscopy (HRMS) was performed using a Xevo G2-XS QTOF (WATERS) mass spectrometer. Scanning electron microscopy (SEM) was performed using a LEO Supra 55 VP (Zeiss) instrument. Powder X-ray diffraction (PXRD) measurements were performed using a Miniflex (Rigaku) diffractometer. Energy-dispersive X-ray spectrometry (EDX) analysis was performed using a JSM-6610-LV (JEOL) instrument. Digital images of the components of the hybrid catalyst were obtained using a smartphone and were utilized for RGB analysis employing the Image J software. Tensile tests were performed using an UTM (INSTRON) tensile tester without an extensometer at 25 °C. The samples used for the tensile tests were 3.20 mm wide and 7.20 mm thick, and their calculated surface area was 23 mm².