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Recombinant murine il 13

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Recombinant murine IL-13 is a cytokine produced in a mammalian cell expression system. It has a molecular weight of approximately 12 kDa.

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Lab products found in correlation

Collagenase a, by Roche (2 mentions) Fetal calf serum (fcs), by Cambrex (2 mentions) Recombinant murine tnfa, by R&D Systems (2 mentions) Recombinant human tgfb, by R&D Systems (2 mentions) Dnase 1, by Merck Group (2 mentions) Recombinant murine il 4, by R&D Systems (2 mentions) Fungizone, by Cambrex (2 mentions) Penicillin, by Cambrex (2 mentions) Streptomycin, by Cambrex (2 mentions)

Spelling variants of this product

IL-13 (210 citations) Recombinant IL-13 (7 citations)

3 protocols using recombinant murine il 13

1

Isolation and Stimulation of Lung Fibroblasts

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The lungs were removed, and single cells were isolated by enzymatic digestion with 1 mg/ml collagenase A (Roche, Indianapolis, IN) and 20 U/ml DNaseI (Sigma, St. Louis, MO) in RPMI 1640 containing 10% FCS. Tissues were further dispersed through an 18-gauge needle (10-ml syringe) and filtered through 100-μm nylon mesh twice. Cells were centrifuged, washed, and cultured in complete medium composed of Dulbecco’s modified Eagle’s medium (Mediatech, Inc.) supplemented with 15% fetal calf serum, 1% L-glutamine, and 100 U/ml penicillin, streptomycin, and fungizone (Cambrex, Walkersville, MD). Cells were used for experiments after one passage. For restimulation, lung fibroblasts were seeded after one passage into a 96 well plate. Lung fibroblasts were restimulated with a cocktail recombinant murine IL-4 (10 ng/ ml;R&D Systems, Minneapolis, USA), recombinant murine IL-13 (10 ng/ml;R&D Systems, Minneapolis, USA), recombinant murine TNFa (5 ng/ml;R&D Systems, Minneapolis, USA) and recombinant human TGFb (10 ng/ml;R&D Systems, Minneapolis, USA) for 24 h for gene expression analysis.
Fonseca W., Rasky A.J., Ptaschinski C., Morris S.H., Best S.K., Phillips M., Malinczak C.A, & Lukacs N.W. (2019). Group 2 innate lymphoid cells (ILC2) are regulated by stem cell factor during chronic asthmatic disease. Mucosal immunology, 12(2), 445-456.
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2

Primary Murine Tracheal Epithelial Cell Culture and Treatment

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Primary murine tracheal epithelial cells (MTBEC) were isolated from B62 mice (obtained from Charles River Laboratories) as previously described.22 (link) For analysis of expression of Th2 cytokines, cells were plated onto collagen (BD Biosciences)–coated coverslips and maintained in MTEC Plus culture medium as described previously.22 (link) Cells were grown until confluent and then exposed to chitosan-coated empty (E) or CaMKIIN (CN) loaded NPs (25, 50 or 100 µg) with recombinant murine IL-13 (10 ng/ml, R&D Systems) for 14 days. RNA was isolated as stated below.
Morris A.S., Sebag S.C., Paschke J.D., Wongrakpanich A., Ebeid K., Anderson M.E., Grumbach I.M, & Salem A.K. (2017). Cationic CaMKII Inhibiting Nanoparticles Prevent Allergic Asthma. Molecular pharmaceutics, 14(6), 2166-2175.
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3

Isolation and Stimulation of Lung Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The lungs were removed, and single cells were isolated by enzymatic digestion with 1 mg/ml collagenase A (Roche, Indianapolis, IN) and 20 U/ml DNaseI (Sigma, St. Louis, MO) in RPMI 1640 containing 10% FCS. Tissues were further dispersed through an 18-gauge needle (10-ml syringe) and filtered through 100-μm nylon mesh twice. Cells were centrifuged, washed, and cultured in complete medium composed of Dulbecco’s modified Eagle’s medium (Mediatech, Inc.) supplemented with 15% fetal calf serum, 1% L-glutamine, and 100 U/ml penicillin, streptomycin, and fungizone (Cambrex, Walkersville, MD). Cells were used for experiments after one passage. For restimulation, lung fibroblasts were seeded after one passage into a 96 well plate. Lung fibroblasts were restimulated with a cocktail recombinant murine IL-4 (10 ng/ ml;R&D Systems, Minneapolis, USA), recombinant murine IL-13 (10 ng/ml;R&D Systems, Minneapolis, USA), recombinant murine TNFa (5 ng/ml;R&D Systems, Minneapolis, USA) and recombinant human TGFb (10 ng/ml;R&D Systems, Minneapolis, USA) for 24 h for gene expression analysis.
Fonseca W., Rasky A.J., Ptaschinski C., Morris S.H., Best S.K., Phillips M., Malinczak C.A, & Lukacs N.W. (2019). Group 2 innate lymphoid cells (ILC2) are regulated by stem cell factor during chronic asthmatic disease. Mucosal immunology, 12(2), 445-456.
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