Mak175

Manufactured by Merck Group

Sourced in Germany

The MAK175 is a versatile laboratory instrument designed for a range of analytical applications. It features a high-precision and robust construction, enabling accurate and reliable measurements. The core function of the MAK175 is to provide users with a reliable tool for their laboratory needs.

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Lab products found in correlation

Mak045, by Merck Group (1 mentions) Mak043, by Merck Group (1 mentions)

2 protocols using mak175

Plasma Lipid Extraction and Quantification

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Extraction of plasma lipids was done with the help of chloroform/methanol mixture (2 : 1, v/v) by following the method described by Yassin et al. [39 (link)]. Total lipid contents in plasma extracts were gravimetrically determined through solvent evaporation with the help of rotary evaporator (BIOLAND RE-5000A China). Parameters of plasma lipids like total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triacylglycerol (TG) levels were calculated through enzymatic method, with the help of commercial kits (TR0100, MAK045, MAK175 Sigma-Aldrich, Germany). Low-density lipoprotein cholesterol (LDL-C) was quantified by the Friedewald equation (Friedewald et al., 1972):

\begin{matrix} LDL ‐ C = TC - (\frac{Triglycerides}{(5 + HDL ‐ C)}) . \end{matrix}

Hussain A., Kausar T., Jamil M.A., Noreen S., Iftikhar K., Rafique A., Iqbal M.A., Majeed M.A., Quddoos M.Y., Aslam J, & Ali A. (2022). In Vitro Role of Pumpkin Parts as Pharma-Foods: Antihyperglycemic and Antihyperlipidemic Activities of Pumpkin Peel, Flesh, and Seed Powders, in Alloxan-Induced Diabetic Rats. International Journal of Food Science, 2022, 4804408.

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Sciatic Nerve Cholesterol Analysis

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Cholesterol was extracted from the sciatic nerves with a cholesterol extraction kit (Sigma-Aldrich; MAK175). Cholesterol contents were analyzed by a kit (Sigma-Aldrich; MAK043).

Zhao Y., Liang Y., Xu Z., Liu J., Liu X., Ma J., Sun C, & Yang Y. (2022). Exosomal miR-673-5p from fibroblasts promotes Schwann cell-mediated peripheral neuron myelination by targeting the TSC2/mTORC1/SREBP2 axis. The Journal of Biological Chemistry, 298(3), 101718.

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