In this study, the immobilized lipases used were Novozym 435 (Candida antarctica lipase B immobilized on an acrylic resin), Lipozyme RM IM (Rhizomucor miehei lipase immobilized on an anionic resin), and Lipozyme TL IM (Themomyces lanuginose lipase immobilized on silica gel). All enzymes were purchased from Novozymes (Bagsvaerd, Denmark). The solvent reactants used were acetonitrile (99.5%), toluene (99.5%), and n-hexane (96%) purchased from Junsei (Tokyo, Japan), as well as acetone (99.5%), tetrahydrofuran (THF), 1,2-dichloroethane (99%), cyclohexane (99.5%), n-heptane (98%), and iso-octane (98%) purchased from Dae-jung (Gyunggido, Korea).
Isooctane
Manufactured by Daejung Chemicals
Isooctane is a volatile, colorless liquid hydrocarbon used as a high-performance fuel in laboratory and industrial applications. It has a chemical formula of C8H18 and serves as a reference fuel for octane rating measurements.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Junsei (2 mentions)
Acetone, by Daejung Chemicals (2 mentions)
Cyclohexane, by Daejung Chemicals (1 mentions)
N heptane, by Daejung Chemicals (1 mentions)
Toluene, by Junsei (1 mentions)
N hexane, by Junsei (1 mentions)
Nile red, by Merck Group (1 mentions)
Sodium thiosulfate, by Merck Group (1 mentions)
Acetic acid, by Daejung Chemicals (1 mentions)
Potassium iodide, by Daejung Chemicals (1 mentions)
Starch, by Merck Group (1 mentions)
Potassium iodide, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Methanol, by Daejung Chemicals (1 mentions)
P anisidine, by Merck Group (1 mentions)
Heptadecanoic acid methyl ester, by Merck Group (1 mentions)
Chloroform, by Daejung Chemicals (1 mentions)
Sodium thiosulfate, by Daejung Chemicals (1 mentions)
Glacial acetic acid, by Daejung Chemicals (1 mentions)
4 protocols using isooctane
1
Immobilized Lipase Catalyzed Reactions
2
Characterization of Emulsion-Based Food Ingredients
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SO and TE powder were obtained from Ottogi Corp., Ltd. (Anyang, Republic of Korea). XG with a viscosity ranging from 1200 to 1600 cPs in a 1% solution in 1% KCl was purchased from Deosen Biochemical (Ordos) Ltd. (Ordos, Inner Mongolia, China). SL was acquired from Solae LLC. (St. Louis, MO, USA). ρ-Anisidine was purchased from Tokyo Chemical Industry Co., Ltd. (Nihonbashi-honcho, Chuo-ku, Tokyo, Japan). Sodium thiosulfate, Nile red, and Calcofluor white dyes were purchased from Sigma-Aldrich (St. Louis, MO, USA). Isooctane, acetic acid, and potassium iodide (KI) were obtained from Daejung Chemicals (Seoul, Republic of Korea). Commercial butter (Anchor butter) was purchased from Fonterra Cooperative Group Ltd. (Auckland, New Zealand). Whole egg liquid and non-fat dry milk were purchased from KC Feed Co., Ltd. (Yeongcheon, Republic of Korea) and Seoul Milk Co. (Seoul, Republic of Korea), respectively. Sugar and flour were purchased from CJ CheilJedang Co., Ltd. (Seoul, Republic of Korea).
3
Lipid Oxidation Analysis Protocol
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Potassium iodide, p-anisidine, potassium hydroxide,
heptadecanoic acid methyl ester, starch, thiobarbituric acid, and
phenolphthalein were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO,
USA). Chloroform, isooctane, glacial acetic acid, methanol, sodium thiosulfate
(0.1 N and 0.01 N), ethanol, and ether were purchased from Daejung Co. (Siheung,
Korea). Benzene and iodine monochloride were purchased from Samchun (Seoul,
Korea). All chemicals were analytical grade and used without further
purification.
heptadecanoic acid methyl ester, starch, thiobarbituric acid, and
phenolphthalein were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO,
USA). Chloroform, isooctane, glacial acetic acid, methanol, sodium thiosulfate
(0.1 N and 0.01 N), ethanol, and ether were purchased from Daejung Co. (Siheung,
Korea). Benzene and iodine monochloride were purchased from Samchun (Seoul,
Korea). All chemicals were analytical grade and used without further
purification.
4
Enzymatic Synthesis of Naringin Ester
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Naringin (95%) and ethyl acetate (99.8%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Novozym 435 (immobilized Candida antarctica lipase B), Lipozyme TL IM (immobilized Thermomyces lanuginosus), and Lipozyme RM IM (immobilized Rhizomucor miehei) were purchased from Novo Nordisk Bioindustry (Bagsvaerd, Denmark). Dimethyl sulfoxide (DMSO) (99.5%), 1,4-dioxane (99.5%), acetone (99.5%), tert-butanol (99.5%), tert-amyl alcohol (99%), 1,2-dichloroethane (99%), isooctane (98%), oleic acid (99%), and molecular sieves (3-Å) were purchased from Dae-Jung Chemical and Metals Co. Ltd. (Gyunggido, Korea). Acetonitrile (99.5%) and tetrahydrofuran (THF) (99.5%) were purchased from Junsei Chemical Co. Ltd. (Tokyo, Japan). Methanol, water, and acetic acid (HPLC grade) were purchased from J.T. Baker (Phillipsburg, NJ, USA).
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