Cells were fixed with 4% PFA for 15 min at room temperature (RT), permeabilized with 0,3% Triton X-100 (in PBS) for 10 min, and then incubated with 1:1000 phalloidin-568 (Thermo) in 3% BSA for 1 hr at RT. After 3x 15 min washes with PBT (0,1% Triton X-100 in PBS), samples were counterstained for 5 min at RT with 1:5000 DAPI (Sigma) and mounted for imaging with Dako Fluorescent mounting medium. Images were obtained on a Zeiss LSM700 confocal microscope using a LD C-Apochromat 63x/1.15 W Corr M27 objective. Protrusion length was measured using ImageJ.
Ld c apochromat 63x 1.15 w corr m27 objective
Manufactured by Zeiss
The LD C-Apochromat 63x/1.15 W Corr M27 objective is a high-performance water immersion objective lens designed for Zeiss microscopes. It provides a magnification of 63x and a numerical aperture of 1.15, enabling high-resolution imaging. The objective is corrected for chromatic aberrations, providing excellent image quality across a wide range of wavelengths. The M27 thread mount allows for easy integration with compatible Zeiss microscope systems.
Automatically generated - may contain errors
2 protocols using ld c apochromat 63x 1.15 w corr m27 objective
1
Actin Cytoskeleton Visualization Protocol
2
Actin Cytoskeleton Visualization Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!