Imaging experiments described in this study were performed as follows unless specifically noted. Epifluorescence imaging experiments were performed on a Leica DMI8 microscope (Semrock bandpass filter: GFP ex/em: FF01–474-27/FF01-520-35, RFP ex/em:FF01-578-21/FF01-600-37) controlled by MetaMorph Imaging software, using a sCMOS camera (Photometrics Prime95B) and 20x magnification lens (Leica HCX PL FLUOTAR L 20x/0.40 NA CORR) or 10× objective (Leica HCX PL FLUOTAR L 10x/0.32 NA). Confocal imaging experiments were performed on a Leica SP8 confocal microscope from the Lynn and Mike Garvey Imaging Core at the Institute of Stem Cell and Regenerative Medicine. Cells were imaged in live cell imaging solution with 10mM glucose (LCIS+, Gibco, A14291DJ). Image analysis methods described below.
Hcx pl fluotar l 10x 0.32 na
Manufactured by Leica
The HCX PL FLUOTAR L 10x/0.32 NA is a microscope objective lens manufactured by Leica. It has a magnification of 10x and a numerical aperture of 0.32.
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2 protocols using hcx pl fluotar l 10x 0.32 na
1
Epifluorescence and Confocal Imaging of Cells
2
Live-cell Fluorescence Microscopy Protocol
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Imaging experiments described in this study were performed as follows unless specifically noted. Epifluorescence imaging experiments were performed on a Leica DMI8 microscope (Semrock bandpass filter: GFP ex/em: FF01–474-27/FF01–520-35, RFP ex/em:FF01–578-21/FF01–600-37) controlled by MetaMorph Imaging software, using a sCMOS camera (Photometrics Prime95B) and 20x magnification lens (Leica HCX PL FLUOTAR L 20x/0.40 NA CORR) or 10× objective (Leica HCX PL FLUOTAR L 10x/0.32 NA). Confocal imaging experiments were performed on a Leica SP8 confocal microscope from the Lynn and Mike Garvey Imaging Core at the Institute of Stem Cell and Regenerative Medicine. Cells were imaged in live cell imaging solution with 10mM glucose (LCIS+, Gibco, A14291DJ). Image analysis methods described below.
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