Ab150089

The coverslips were washed with PBS twice and the cells were fixed using 4% formaldehyde overnight, washed thrice in PBS and blocked in blocking buffer (1× PBS/5% normal serum/0.3% Triton™ X-100) for 60 min. The coverslips were incubated with antibody dilution (diluted in 1× PBS/1% BSA/0.3% Triton™ X-100) for overnight at 4 °C (PERK ab (AF3999-SP, Novus Biologicals, Littleton, USA)), GM130 ab (#610822, BD Biosciences, US), Calnexin ab (#AD1-SPA-860-D, EnzoLifeSciences GmbH, Germany)), rinsed thrice with PBS and incubated with Fluorochrome-conjugated secondary antibody for 1–2 h at room temperature in the dark and humid environment. Anti-goat (ab150089, Abcam, Cambridge, UK), anti-rabbit (a10042, ThermoFisher Scientific, Massachusetts, US) and anti-mouse (ab150113, Abcam, Cambridge, UK) secondary antibodies were used having Alexa488, Alexa568 and Alexa488 labels respectively. After secondary antibody incubation the coverslips were mounted with Vectorsheild DAPI-mount (#H-1200) onto a glass slide. The images were taken using a Leica SP8 confocal microscope.

The following primary antibodies were used: rat anti-CTIP2 1:500 (Abcam ab18465), rabbit anti-CUX-1 1:500 (Abcam, ab140042), rabbit anti-IBA-1 primary antibody (Abcam, ab178846), rabbit anti-IBA-1 primary antibody 1:500 (Wako, 4987481428584), rabbit anti-Caspr2 1:1000 (Abcam ab137052), rabbit MAPK1/ERK antibodies 1:1000 (Cell Signalling mAb#4695), rabbit anti-DSred primary antibody 1:500 (Takara, 632496) which binds Td-tomato protein, and guinea pig anti-VGLUT-1 1:1000 (Synaptic Systems 135 304).
The following secondary antibodies were used: goat anti-rabbit Alexa-488 secondary antibody 1:1000 (Abcam, ab150089), donkey anti-rabbit Alexa-488 1:1000 (Abcam ab150073), goat anti-guinea pig Alexa-647 secondary antibody 1:1000 (Abcam ab150187), donkey anti-rabbit Alexa-647 1:1000 (ABCAM ab150075), goat anti-rat Alexa-488 1:1000, (Abcam ab150157).