Annexin 5 fitc kit

HSYA (C₂₇H₃₂O₁₆, purity >92.5%) was obtained from China Pharmaceutical and Biological Products, Beijing, CN), and GW9662 and RGZ (purity >99%) were purchased from Abcam. Human gastric carcinoma BGC-823 cells were purchased from the Cancer Hospital of the Chinese Academy of Medical Sciences. Dulbecco’s minimal essential medium (DMEM), fetal bovine serum (FBS), and streptomycin-penicillin were from Hyclone. MTT, dimethyl sulfoxide (DMSO), and annexin V-FITC Kit were from Solarbio. PPARγ and caspase-3 antibodies were purchased from CST. Secondary antibodies were purchased from ComWin Biotech. DAPI was obtained from Biotopped and Trizol was obtained from Invitrogen. The PCR kit was purchased from Roche.

High-glucose Dulbecco’s modified Eagle medium (DMEM), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), frozen dimethyl sulfoxide (DMSO) stock solution, trypsin, TRIzol, RIPA, BCA kit and Annexin V-FITC kit were purchased from Solarbio (Beijing, China). Fetal bovine serum (FBS), penicillin, streptomycin, and phosphate-buffered saline (PBS) were purchased from HyClone (Logan，UT，USA). PrimeScript™ RT reagent kit was purchased from TaKaRa (Japan). Ultrapure water was purchased from Gen Pure (Germany), and 30% H₂O₂ was purchased from Nanjing Reagent (Nanjing,China). Lactate dehydrogenase (LDH), superoxide dismutase (SOD), aspartate aminotransferase (AST), creatine kinase (CK), and glutathione peroxidase (GSH-PX) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). A PrimeScriptRT reagent kit (Perfect Real Time) was obtained from TaKaRa Biotechnology Co. (Japan). Antibodies against Akt1, p-Akt1, GSK-3β, and GAPDH were obtained from Affinity Biosciences (Cincinnati, OH, USA). Secondary antibodies were purchased from Beyotime (Shanghai, China).