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Fam dye labeled probes

Manufactured by Thermo Fisher Scientific
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FAM dye labeled probes are fluorescent oligonucleotides used in quantitative PCR (qPCR) and other nucleic acid detection applications. The FAM dye, which emits green fluorescence, is covalently attached to the probe sequence, allowing for the detection and quantification of target DNA or RNA molecules.

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Lab products found in correlation

High capacity rna to cdna kit, by Thermo Fisher Scientific (4 mentions) Vic dye labeled probe, by Thermo Fisher Scientific (2 mentions) Quantstudio 6 flex, by Thermo Fisher Scientific (2 mentions) Rneasy kit, by Qiagen (2 mentions)

Close spelling variants of this product

FAM dye-labeled TaqMan probes FAM dye-labeled MGB-probes TaqMan MGB probe (6-FAM dye-labeled), TaqMan Gene Expression Assays (FAM dye-labeled MGB probe; FAM dye-labeled TaqMan MGB probes FAM-labeled probes FAM Dye

2 protocols using fam dye labeled probes

1

Gene Expression Analysis by qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using a RNeasy kit (QIAGEN) and reverse-transcription was done using the High Capacity RNA-to-cDNA kit (Thermo Fisher). Real-time quantitative PCR was done on a QuantStudio 6 Flex (Thermo Fisher) using TaqMan PCR (Applied Biosciences). FAM dye labeled probes (Thermo Fisher) were used against KRAS, CHEK1, MYC, and RIF1 and a VIC dye labeled probe was against ACTB, as a house-keeping control. Delta-delta CT was calculated for each sample. All samples were run as technical duplicates which were then standardized to their respective NS or vehicle treated controls. Means and standard deviations were calculated from the standardized values of three to four biological replicates.
Klomp J.E., Lee Y.S., Goodwin C.M., Papke B., Klomp J.A., Waters A.M., Stalnecker C.A., DeLiberty J.M., Drizyte-Miller K., Yang R., Diehl J.N., Yin H.H., Pierobon M., Baldelli E., Ryan M.B., Li S., Peterson J., Smith A.R., Neal J.T., McCormick A.K., Kuo C.J., Counter C.M., Petricoin EF I.I.I., Cox A.D., Bryant K.L, & Der C.J. (2021). CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell reports, 37(9), 110060.
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2

Gene Expression Analysis by qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using a RNeasy kit (QIAGEN) and reverse-transcription was done using the High Capacity RNA-to-cDNA kit (Thermo Fisher). Real-time quantitative PCR was done on a QuantStudio 6 Flex (Thermo Fisher) using TaqMan PCR (Applied Biosciences). FAM dye labeled probes (Thermo Fisher) were used against KRAS, CHEK1, MYC, and RIF1 and a VIC dye labeled probe was against ACTB, as a house-keeping control. Delta-delta CT was calculated for each sample. All samples were run as technical duplicates which were then standardized to their respective NS or vehicle treated controls. Means and standard deviations were calculated from the standardized values of three to four biological replicates.
Klomp J.E., Lee Y.S., Goodwin C.M., Papke B., Klomp J.A., Waters A.M., Stalnecker C.A., DeLiberty J.M., Drizyte-Miller K., Yang R., Diehl J.N., Yin H.H., Pierobon M., Baldelli E., Ryan M.B., Li S., Peterson J., Smith A.R., Neal J.T., McCormick A.K., Kuo C.J., Counter C.M., Petricoin EF I.I.I., Cox A.D., Bryant K.L, & Der C.J. (2021). CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell reports, 37(9), 110060.
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