PLX4032, AMG-510, and MRTX-1257 were dissolved in 2% DMSO + 30% PEG 300 + 5% Tween 80 + ddH2O. Tumors were engrafted in NSG (NOD-SCID) mice (The Jackson Laboratory) by subcutaneous injection of 3 × 105 A375 or H2122 cells in RPMI-1640 medium supplemented with 50% Matrigel (BD Biosciences, cat. no. 354234). Seven days after the injection, animals were assigned randomly to control and various treatment groups (n = 5 for each group). Tumor bearing mice were administered by oral garvage: (1) Vehicle, 2% DMSO + 30% PEG 300 + 5% Tween 80 + ddH2O; (2) PLX4032, 1 mg/kg body weight/day; (3) Atorvastatin, 1.2 mg/kg body weight/day; (4) AMG-510, 5 mg/kg body weight/day; (5) MRTX-1257, 3 mg/kg body weight/day. The mice were treated every other day. Tumors were measured with an external caliper, and the volume was calculated as (4π/3) × (width/2)2 × (length/2).
Nsg nod scid mice
Manufactured by Jackson ImmunoResearch
NSG (NOD-SCID) mice are a strain of immunodeficient mice developed for use in biomedical research. They lack mature T cells, B cells, and natural killer cells, making them suitable for engraftment of human cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nsg nod scid mice
1
Evaluating KRAS and BRAF Inhibitors in Mice
2
Evaluating Anti-Cancer Drug Efficacy
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PLX4032, AMG-510, and MRTX-1257 were dissolved in 2% DMSO + 30% PEG 300 + 5% Tween 80 + ddH2O. Tumors were engrafted in NSG (NOD-SCID) mice (The Jackson Laboratory) by subcutaneous injection of 3 × 10 5 A375 or H2122 cells in RPMI-1640 medium supplemented with 50% Matrigel (BD Biosciences, cat. no. 354234). Seven days after the injection, animals were assigned randomly to control and various treatment groups (n = 5 for each group). Tumor bearing mice were administered by oral garvage: (1) Vehicle, 2% DMSO + 30% PEG 300 + 5% Tween 80 + ddH2O; (2) PLX4032, 1 mg/kg body weight/day; (3) Lipitor, 1.2 mg/ kg body weight/ day; (4) AMG-510, 5 mg /kg body weight/day; (5) MRTX-1257, 3 mg/ kg body weight/day. The mice were treated every other day. Tumors were measured with an external caliper, and the volume was calculated as (4π/3) × (width/2) 2 × (length/2).
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