The largest database of trusted experimental protocols

2 protocols using leukotriene b4

1

Eicosanoid Regulation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aspirin (ASP: 2-acetoxybenzoic acid), dexamethasone (DEX: (11β,16α)-9-fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3), ibuprofen (IBU: α-methyl-4-isobutyl phenylacetic acid), prostaglandin E2 (PGE2: (5Z,11α,13E,15S-11,15-dihydroxy-9-oxoprosta-5,13-dienoic acid), naproxen (NAP: 6-methoxy-α-methyl-2-naphthaleneacetic acid), leukotriene B4 (LTB4: 5,12-dihydroxy-6,8,10,14-eicosatetraenoic acid), and 14,15-epoxyeicosatrienoic acid (14,15-EET) were purchased from Sigma-Aldrich, Korea (Seoul, Republic of Korea), and dissolved in dimethyl sulfoxide (DMSO, 10%). Antibody specific to PGE2 was purchased from Abcam (ab2318, Abcam, Cambridge, UK), and its secondary antibody conjugated with fluorescence isothiocyanate (FITC) was purchased from Thermo Fisher Scientific, Korea (Seoul, Republic of Korea).
+ Open protocol
+ Expand
2

Comprehensive Metabolite Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetylcholine, acetyl-d3-L-carnitine hydrochloride, acetyl-Lcarnitine, adrenic acid, L-carnitine, Lcitrulline, dodecanoic acid, (-)-epicatechin, gallic acid, glycochenodeoxycholic acid, glycocholic acid, glycocholic acid-(glycyl-1-13C) monohydrate, α-hydroxyisobutyric acid, indole-3-acetic-2,2-d2 acid, Lisoleucine, 7-ketocholesterol, L-leucine, leukotriene B4, margaric acid, palmitic acid, Lphenylalanine, stearic acid, syringic acid, L-tryptophan, uric acid, and L-valine were purchased from Sigma-Aldrich (St. Louis, MO). 4-hydroxyhippuric acid was purchased from PhytoLab GmbH and Co KG (Vestenbergsgreuth, Germany), naringenin from Extrasynthèse (Genay, France), and arachidonic acid from Cymit Quimica (Barcelona, Spain). UHPLC-MS-grade methanol, acetone, formic acid, and HPLC-grade acetonitrile were purchased from Scharlau Chemie S.A. (Barcelona, Spain). Ultrapure water (Milli-Q) was obtained from a Milli-Q Gradient A10 system (Millipore, Bedford, MA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!