We collected treated monocytes and lysed them with RIPA buffer. Electrophoresis with 8%-12% SDS-polyacrylamide gel was used to separate proteins. We then transferred the proteins onto a PVDF membrane (Millipore, Bedford, MA). After blocking the membrane with 2% BSA for 1 hour at room temperature, we incubated the membrane overnight at 4°C with the primary antibodies against NLRP3 (ab263899, Abcam), cleaved caspase-1 (D57A2, Cell Signaling Technology), GCN2 (ab134053, Abcam), phospho-GCN2 (ab75836, Abcam) and β-actin (ab8227, Abcam). We probed the membranes with horseradish peroxidase-conjugated secondary antibodies, and then developed the membranes with an enhanced chemiluminescence system.
Ab134053
Manufactured by Abcam
Ab134053 is a recombinant antibody product manufactured by Abcam. It is a purified protein designed for use in laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab75836, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ab8227, by Abcam (1 mentions)
Ab263899, by Abcam (1 mentions)
Ab16288, by Abcam (1 mentions)
Ab109250, by Abcam (1 mentions)
Ab200834, by Abcam (1 mentions)
Ab16285, by Abcam (1 mentions)
Ficoll paque premium sterile solution, by GE Healthcare (1 mentions)
Af1738, by Beyotime (1 mentions)
Af938, by R&D Systems (1 mentions)
Y 27632, by STEMCELL (1 mentions)
Cytotune ips 2.0 sendai reprogramming kit, by Thermo Fisher Scientific (1 mentions)
Matrigel, by BD (1 mentions)
Accutase, by STEMCELL (1 mentions)
Mtesr medium, by STEMCELL (1 mentions)
2 protocols using ab134053
1
Western Blot Analysis of Inflammatory Proteins
2
Antibody Profiling for Stem Cell Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were used: goat antibody against human VE-cadherin (1:1,000, AF938; R&D systems); rabbit antibody against human GCN2 (1:1,000; ab134053; Abcam); rabbit antibody against human NANOG (1:500; ab109250; Abcam); rabbit antibody against human OCT4 (1:500; ab200834; Abcam); mouse antibody against human SSEA-1 (1:500; ab16285; Abcam); mouse antibody against human TRA-1 (1:500; ab16288; Abcam); rabbit antibody against phospho-Akt (Ser473) (1:1,000; 31957; Cell Signaling); rabbit antibody against Akt (pan) (1:2,000; 4691; Cell Signaling); Ki67 rabbit monoclonal antibody (1:200; AF1738, Beyotime); Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1:1,000; R37118; Life Technologies); Ficoll-Paque PREMIUM sterile solution (17544202; GE Healthcare); CytoTune-iPS 2.0 Sendai Reprogramming Kit (A16517; Thermo Fisher Scientific); Matrigel (354277; BD); mTeSR medium (05850; STEMCELL Technologies); Y-27632 (STEMCELL Technologies); Accutase (07920; STEMCELL Technologies); MTT for cell proliferation detection (C0009S; Beyotime).
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