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Anti cd31 percp

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Anti-CD31-PerCP is a lab equipment product that detects the CD31 cell surface marker. CD31, also known as PECAM-1, is a glycoprotein expressed on the surface of various cell types, including endothelial cells, platelets, and certain leukocytes. The Anti-CD31-PerCP product uses the PerCP (Peridinin Chlorophyll Protein Complex) fluorescent label to enable the identification and enumeration of CD31-positive cells through flow cytometry analysis.

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Lab products found in correlation

Anti cd45 apc, by BD (2 mentions) Anti sca 1, by BioLegend (2 mentions) Anti cdh5 alexa fluor 647, by BD (2 mentions) Anti pdgfrα apc, by Thermo Fisher Scientific (2 mentions) Anti cd34 apc, by BioLegend (2 mentions) Anti cd44 percp, by BioLegend (2 mentions) Lsr fortessa 2, by BD (2 mentions) Syto 16, by Thermo Fisher Scientific (2 mentions) Accuri c6, by BD (2 mentions) Anti cd11b pe, by BioLegend (2 mentions) Anti cd117 pe, by BioLegend (2 mentions) Anti cd29 pe, by BD (2 mentions)

Spelling variants of this product

Anti-CD31 (45 citations) Anti-mouse CD31-PerCP-Cy5.5 (2 citations) PerCP/Cy5.5 anti-CD31 (2 citations)

2 protocols using anti cd31 percp

1

Multiparametric Flow Cytometry of Adipose-derived Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary cells obtained after the enzymatic digestion of periaorta adipose tissue or cultured cells detached with scraptase (GenDEPOT) were stained for 30 minutes at 4°C with following antibodies: anti-CD45-APC (BD Biosciences, 561018), anti-CD29-PE (BD Biosciences, 562801), anti-Sca-1 (stem cell antigen 1)-PE-Cy7 (Biolegend, 108113), anti-CD31-PerCP (Biolegend, 201419), anti-CDH5-Alexa Fluor 647 (BD Biosciences, 562242), anti-PDGFRα-APC (platelet-derived growth factor α; eBioscience, 17-1401-81), anti-CD117-PE (Biolegend, 105807), anti-CD34-APC (Biolegend, 128611), anti-CD44-PerCP (Biolegend, 103035), and anti-CD11b-PE (Biolegend, 101205). Nucleated cells were distinguished from debris with Syto16 (Molecular Probes, S7578) and dead cells with 4′,6-diamidino-2-phenylindole (DAPI). Cells were analyzed with BD Accuri C6 or BD LSR Fortessa II (both Becton Dickinson). Gating was set with appropriate fluorescence minus one controls or corresponding IgG controls.
Gu W., Nowak W.N., Xie Y., Le Bras A., Hu Y., Deng J., Issa Bhaloo S., Lu Y., Yuan H., Fidanis E., Saxena A., Kanno T., Mason A.J., Dulak J., Cai J, & Xu Q. (2019). Single-Cell RNA-Sequencing and Metabolomics Analyses Reveal the Contribution of Perivascular Adipose Tissue Stem Cells to Vascular Remodeling. Arteriosclerosis, Thrombosis, and Vascular Biology, 39(10), 2049-2066.
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2

Multiparametric Flow Cytometry of Adipose-derived Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary cells obtained after the enzymatic digestion of periaorta adipose tissue or cultured cells detached with scraptase (GenDEPOT) were stained for 30 minutes at 4°C with following antibodies: anti-CD45-APC (BD Biosciences, 561018), anti-CD29-PE (BD Biosciences, 562801), anti-Sca-1 (stem cell antigen 1)-PE-Cy7 (Biolegend, 108113), anti-CD31-PerCP (Biolegend, 201419), anti-CDH5-Alexa Fluor 647 (BD Biosciences, 562242), anti-PDGFRα-APC (platelet-derived growth factor α; eBioscience, 17-1401-81), anti-CD117-PE (Biolegend, 105807), anti-CD34-APC (Biolegend, 128611), anti-CD44-PerCP (Biolegend, 103035), and anti-CD11b-PE (Biolegend, 101205). Nucleated cells were distinguished from debris with Syto16 (Molecular Probes, S7578) and dead cells with 4′,6-diamidino-2-phenylindole (DAPI). Cells were analyzed with BD Accuri C6 or BD LSR Fortessa II (both Becton Dickinson). Gating was set with appropriate fluorescence minus one controls or corresponding IgG controls.
Gu W., Nowak W.N., Xie Y., Le Bras A., Hu Y., Deng J., Issa Bhaloo S., Lu Y., Yuan H., Fidanis E., Saxena A., Kanno T., Mason A.J., Dulak J., Cai J, & Xu Q. (2019). Single-Cell RNA-Sequencing and Metabolomics Analyses Reveal the Contribution of Perivascular Adipose Tissue Stem Cells to Vascular Remodeling. Arteriosclerosis, Thrombosis, and Vascular Biology, 39(10), 2049-2066.
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