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Pex vector

Manufactured by GenePharma
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Sourced in China

The PEX vector is a plasmid-based tool used for gene expression in mammalian cells. It provides a robust and efficient platform for the introduction and expression of genetic constructs in various cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Si dbf4, by GenePharma (2 mentions) Pex dbf4 overexpression vector, by GenePharma (2 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (2 mentions) Mutated vector, by Keygen Biotech (2 mentions)

Spelling variants of this product

PEX-3 vector (20 citations) PEX-2 vector (9 citations) PEX-1 vector (5 citations) PEX-3 (pGCMV/MCS/Neo) vector (2 citations) PEX-DBF4 overexpression vector (2 citations)

2 protocols using pex vector

1

Knockdown and Overexpression of DBF4

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Small interfering RNA (siRNA) targeting DBF4 (si-DBF4), negative control siRNA (si-NC), miR-30a mimics with corresponding negative controls (NC), the PEX-DBF4 overexpression vector, and the PEX vector were purchased from Genepharma (Shanghai, China). The mutated vector was constructed by KeyGEN BioTECH (Jiangsu, China). All of the constructs were verified by sequencing. Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) was used to perform cell transfection, in accordance with the manufacturer’s protocol.
Wang T., Ji R., Liu G., Ma B., Wang Z, & Wang Q. (2021). Lactate induces aberration in the miR-30a–DBF4 axis to promote the development of gastric cancer and weakens the sensitivity to 5-Fu. Cancer Cell International, 21, 602.
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2

Targeted Gene Silencing and Overexpression

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Small interfering RNA (siRNA) targeting DBF4 (si-DBF4), negative control siRNA (si-NC), miR-30a mimics with corresponding negative controls (NC), the pEX-DBF4 overexpression vector, and the pEX vector were purchased from Genepharma (Shanghai, China). The mutated vector was constructed by KeyGEN BioTECH (Jiangsu, China). All of the constructs were veri ed by sequencing. Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) was used to perform cell transfection, in accordance with the manufacturer's protocol.
Wang T., Ji R., Liu G., Ma B., Wang Z., & Zuo X. (2021). Lactate Induces Aberration in the miR-30a-DBF4 Axis to Promote the Development of Gastric Cancer and Weakens the Sensitivity to 5-Fu.
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