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2 protocols using nicotine adenine dinucleotide phosphate reduced salt nadph

1

Antioxidant Evaluation of Epicatechin

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Tert-butylhydroperoxide (t-BOOH), (−)-epicatechin (EC, >95% of purity), Glutathione reductase (GR), reduced glutathione (GSH) and oxidized glutathione, dichlorofluorescin (DCFH), o-phthaldialdehyde (OPT), nicotine adenine dinucleotide phosphate reduced salt (NADPH), gentamicin, penicillin G and streptomycin were purchased from Sigma-Aldrich Chemical (Madrid, Spain). Bradford reagent was from BioRad Laboratories S.A (Madrid, Spain). Methanol of HPLC grade, dimethyl sulfoxide (DMSO), light petroleum and phosphoric acid of analytical grade were purchased by Panreac (Barcelona, Spain). DMEM culture media and foetal bovine serum (FBS) were from Cultek (Madrid, Spain). All other reagents were of analytical quality.
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2

Biochemical Assays for Oxidative Stress

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Tert-butylhydroperoxide (t-BOOH), GR, reduced and oxidized (GSSG) glutathione, di-chlorofluorescin (DCFH), o-phthaldialdehyde (OPT), nicotine adenine dinucleotide phosphate reduced salt (NADPH), 2,4-dinitrophenylhydrazine (DNPH), H2O2, 1,1,3,3-tetraethoxypropane (TEP), gentamicin, penicillin G and streptomycin were purchased from Sigma Chemical Co. (Madrid, Spain). Acetonitrile, methanol of HPLC grade, dimethyl sulfoxide (DMSO) of analytical grade and all other usual laboratory reagents were acquired from Panreac (Barcelona, Spain). Bradford reagent was from BioRad Laboratories S.A. DMEM culture media and fetal bovine serum (FBS) were from Cultek (Madrid, Spain). All other reagents were of analytical quality.
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