K2[PtCl4], DMSO, di(2-picolyl)amime, piperidine-1-sulfonyl chloride, 1-naphthalene sulfonyl chloride, 2-naphthalene sulfonyl chloride, analytical grade dioxane, analytical grade methanol, chromasolv water, dichloromethane, anhydrous sodium sulphate and acetonitrile were used as received from Sigma Aldrich, USA. Human breast cancer cell line, MCF-7, was obtained from American Type Culture Collection.
Chromasolv water
Manufactured by Merck Group
Sourced in United States
Chromasolv water is a high-purity solvent designed for use in analytical and chromatographic applications. It is a clear, colorless liquid that meets the specifications for reagent-grade water.
Automatically generated - may contain errors
Lab products found in correlation
Analytical grade methanol, by Merck Group (1 mentions)
Anhydrous sodium sulphate, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Speedvac concentrator, by Eppendorf (1 mentions)
Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Gel doc xr system, by Bio-Rad (1 mentions)
Nucleospin plant 2 kit, by Macherey-Nagel (1 mentions)
2 protocols using chromasolv water
1
Platinum Complex-based Breast Cancer Therapy
2
Genomic DNA Extraction and Characterization
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Five days after anthesis, the tenth leaf (numbered from the bottom to the top of the plant) of 3 plants per genotype (field experiment) were harvested, frozen in liquid nitrogen and stored at -80 °C until use. Genomic DNA was isolated from 100 μg of ground frozen samples using the NucleoSpin Plant II ® kit (Macherey-Nagel, Düren, Germany), following the manufacturer's instructions.
DNA was quantified in a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and the concentration was adjusted to 4 μg μl -1 by diluting with Chromasolv ® water (Sigma-Aldrich, St. Louis, MO, USA) or concentrating with a Speed vac concentrator (Eppendorf AG, Hamburg, Germany).
The DNA from each sample was separated on a 0.8% (w/v) and 1.5% (w/v) agarose gel at 35 V for 20 h and stained with ethidium bromide. Images were obtained by a Gel Doc XR+ System (Bio-Rad, CA, USA).
DNA was quantified in a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and the concentration was adjusted to 4 μg μl -1 by diluting with Chromasolv ® water (Sigma-Aldrich, St. Louis, MO, USA) or concentrating with a Speed vac concentrator (Eppendorf AG, Hamburg, Germany).
The DNA from each sample was separated on a 0.8% (w/v) and 1.5% (w/v) agarose gel at 35 V for 20 h and stained with ethidium bromide. Images were obtained by a Gel Doc XR+ System (Bio-Rad, CA, USA).
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