Cells (at a confluence of 65–70%) were treated with BFA (10 μM) for 1 or 4 h. After treatment, cells were washed twice with PBS and lysed or were left to recover in fresh growth medium for 2 h before lysis. The lysates were subjected to WB with anti-APP C-terminal antibody (Sigma).
Anti app c terminal antibody
Manufactured by Merck Group
Sourced in United States
The Anti-APP C-terminal antibody is a laboratory reagent used for the detection and analysis of the C-terminal region of the amyloid precursor protein (APP). This antibody can be utilized in various immunoassay techniques, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), to study the expression and localization of APP in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Merck Group (2 mentions)
Anti gapdh, by Merck Group (2 mentions)
Anti gfap, by Abcam (2 mentions)
Anti insulin degrading enzyme, by Santa Cruz Biotechnology (2 mentions)
Anti neprilysin, by R&D Systems (2 mentions)
Clemastine, by Bio-Techne (2 mentions)
Anti bace1, by Abcam (2 mentions)
Anti iba1, by Abcam (2 mentions)
3 protocols using anti app c terminal antibody
1
Investigating APP C-terminal Regulation
2
Clemastine Modulates APP Processing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-Aβ (6E10, Convance, USA), anti-FL-APP (CST, USA), anti-APP C-terminal antibody(Sigma-Aldrich, USA), anti-BACE1 (Abcam, USA), anti-GFAP (Abcam), anti-Iba1(Abcam), anti-mTOR (CST), anti-p-mTOR (CST), anti-P70S6K (CST), anti-p-P70S6K (CST), anti-LC3 (CST), anti-p62 (CST), anti-GAPDH (Sigma-Aldrich), anti-insulin degrading enzyme (IDE) (Santa Cruz, USA), anti-neprilysin (R&D, USA). Alexa Fluorconjugated secondary antibodies were from Invitrogen. Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Sigma-Aldrich. Drug treatments 4-month old APP/PS1 transgenic mice and the age-matched WT mice were received a diet of standard laboratory chow supplemented with clemastine (10 mg/kg/day) (sodium salt; Tocris Bioscience, Bristol, Britain) for 4 months. The transgenic mice and WT mice received the same chow without clemastine.
Cell lines were treated with clemastine (dissolved in DMSO) at 0.3, 3, and 30 μM for 12 h. Cells treated with DMSO served as control.
Cell lines were treated with clemastine (dissolved in DMSO) at 0.3, 3, and 30 μM for 12 h. Cells treated with DMSO served as control.
3
Clemastine Modulates APP Processing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-Aβ (6E10, Convance, USA), anti-FL-APP (CST, USA), anti-APP C-terminal antibody(Sigma-Aldrich, USA), anti-BACE1 (Abcam, USA), anti-GFAP (Abcam), anti-Iba1(Abcam), anti-mTOR (CST), anti-p-mTOR (CST), anti-P70S6K (CST), anti-p-P70S6K (CST), anti-LC3 (CST), anti-p62 (CST), anti-GAPDH (Sigma-Aldrich), anti-insulin degrading enzyme (IDE) (Santa Cruz, USA), anti-neprilysin (R&D, USA). Alexa Fluorconjugated secondary antibodies were from Invitrogen. Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Sigma-Aldrich. Drug treatments 4-month old APP/PS1 transgenic mice and the age-matched WT mice were received a diet of standard laboratory chow supplemented with clemastine (10 mg/kg/day) (sodium salt; Tocris Bioscience, Bristol, Britain) for 4 months. The transgenic mice and WT mice received the same chow without clemastine.
Cell lines were treated with clemastine (dissolved in DMSO) at 0.3, 3, and 30 μM for 12 h. Cells treated with DMSO served as control.
Cell lines were treated with clemastine (dissolved in DMSO) at 0.3, 3, and 30 μM for 12 h. Cells treated with DMSO served as control.
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