Cells (105) were inoculated in each well of the eight-chamber slide (Millicell EZ slide, Millipore Corp., Billerica, MA, USA) and were incubated at the two different pressures serially for 0 h, 3 h, 6 h, 9 h, and 12 h. A pipette tip was used to create a scratch wound in each well. The cells were stained with primary rabbit monoclonal anti-E-cadherin, anti-β-catenin, and anti-acetylated α-tubulin antibodies (Cell Signaling Technology Inc., Boston, MA, USA). Fluorescein isothiocyanate-conjugated AffiniPure Goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) was used as the secondary antibody. Nuclei were counterstained with mounting medium (Vector Laboratories Inc., Burlingame, CA, USA) containing 4′,6-diamidino-2-phenylindole. The stained cells were examined with a confocal microscopic examination at a magnification of ×1000 (Leica TCS SP2, Leica Microsystems Inc., Buffalo Grove, IL, USA).
Fluorescein isothiocyanate conjugated affinipure goat anti rabbit igg
Manufactured by Jackson ImmunoResearch
Sourced in United States
Fluorescein isothiocyanate-conjugated AffiniPure Goat anti-rabbit IgG is a secondary antibody reagent. It is conjugated to the fluorescent dye fluorescein isothiocyanate (FITC) and is specific for rabbit immunoglobulin G (IgG).
Automatically generated - may contain errors
Lab products found in correlation
Mounting medium, by Vector Laboratories (1 mentions)
Anti β catenin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Millicell ez slide, by Merck Group (1 mentions)
Leica tcs sp2, by Leica Microsystems (1 mentions)
Lipofectamine 2000, by Wisent (1 mentions)
Peroxidase conjugated affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
2 protocols using fluorescein isothiocyanate conjugated affinipure goat anti rabbit igg
1
Spatial Dynamics of Cell-Cell Adhesion Proteins
2
Antibody Reagents for Cell Adhesion Studies
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Mouse anti‐vinculin (clone V9131), rabbit anti‐paxillin (SAB4502553), rabbit anti‐integrin α3 (AB1920), and rabbit anti‐actin IgGs (A2066) were purchased from Millipore‐Sigma (Oakville, ON). Mouse anti‐human talin‐1 IgG (clone 97H6) was purchased from Bio‐Rad Laboratories (Mississauga, ON). Rabbit monoclonal antibody to phospho‐FAK Y397 (31H5L17) was purchased from ThermoFisher Scientific (Saint‐Laurent, QC). Rat anti‐collagen IV α5 clone H53 (7078) was purchased from Chondrex Inc. (Woodinville, WA). Rabbit anti‐nephrin antiserum was kindly provided by Dr. Tomoko Takano (McGill University). Fluorescein isothiocyanate‐conjugated AffiniPure goat anti‐rabbit IgG (#111‐095‐003), rhodamine‐conjugated AffiniPure goat anti‐mouse IgG (#415‐505‐1), peroxidase‐conjugated AffiniPure sheep‐anti mouse IgG (#515‐035‐003), and peroxidase‐conjugated AffiniPure goat anti‐rabbit IgG (#111‐035‐003) were purchased from Jackson Immunoresearch (West Grove, PA).
Electrophoresis reagents were from Bio‐Rad Laboratories (Mississauga, ON), and GE Healthcare (Baie d'Urfé, QC). Tissue culture media and Lipofectamine 2000 were from Wisent (Saint‐Jean‐Baptiste, QC) and Invitrogen‐Life Technologies (Burlington, ON).
Electrophoresis reagents were from Bio‐Rad Laboratories (Mississauga, ON), and GE Healthcare (Baie d'Urfé, QC). Tissue culture media and Lipofectamine 2000 were from Wisent (Saint‐Jean‐Baptiste, QC) and Invitrogen‐Life Technologies (Burlington, ON).
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