L glut

Manufactured by Euroclone

Sourced in Italy

L-Glut is a laboratory reagent used as a source of L-glutamine, an essential amino acid. It is commonly used in cell culture media to support the growth and maintenance of various cell lines. The product provides a stable form of L-glutamine to supplement culture conditions.

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Lab products found in correlation

Fetal bovine serum (fbs), by Euroclone (2 mentions) Penicillin streptomycin, by Euroclone (1 mentions) Pen strep, by Euroclone (1 mentions) Foetal bovine serum (fbs), by Euroclone (1 mentions) Rpmi 1640 medium, by Euroclone (1 mentions) Hct116 cell, by American Type Culture Collection (1 mentions) Ecb3054d, by Euroclone (1 mentions) Ecb7501l, by Euroclone (1 mentions)

3 protocols using l glut

Human IGR39 Melanoma Cell Culture

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Human IGR39 melanoma cells were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Cells were cultured in standard condition in DMEM (Dulbecco’s Modified Eagle’s Medium-high glucose, Cat. No ECB7501L, EuroClone, Italy) supplemented with 10% fetal bovine serum (FBS, Cat. No ECS0180D, EuroClone, Italy), 1% non-essential amminoacids (NEA, Cat. No ECB3054D, EuroClone, Italy), 1% antibiotics (Penicillin/Streptomycin, Pen/Strep, Cat. No ECB3001D, EuroClone, Italy), 1% L-Glutamine (L-Glut, Cat. No ECB3000D-20, EuroClone, Italy) (complete medium) at

37^{\circ}

C and 5% CO

_{2}

and 95% humidity.

Lionetti M.C., Cola F., Chepizhko O., Fumagalli M.R., Font-Clos F., Ravasio R., Minucci S., Canzano P., Camera M., Tiana G., Zapperi S, & Porta C.A. (2020). MicroRNA-222 Regulates Melanoma Plasticity. Journal of Clinical Medicine, 9(8), 2573.

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Culture of CRC Cell Lines

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All the CRC cell lines were cultured in RPMI-1640 medium (Euroclone; Milan, Italy), supplemented with 2% L-Glut, 10% foetal bovine serum (Euroclone) and 1% penicillin/streptomycin (complete medium). HCT-116 cells were obtained from the American Type Culture Collection ATCC (Manassas, VA, USA); HT-29 cells were kindly provided by Dr R Falcioni (Regina Elena Cancer Institute, Roma, Italy); HCT-8 and H630 were kindly provided by Dr E Mini (University of Florence, Florence, Italy).

Pillozzi S., D'Amico M., Bartoli G., Gasparoli L., Petroni G., Crociani O., Marzo T., Guerriero A., Messori L., Severi M., Udisti R., Wulff H., Chandy K.G., Becchetti A, & Arcangeli A. (2017). The combined activation of KCa3.1 and inhibition of Kv11.1/hERG1 currents contribute to overcome Cisplatin resistance in colorectal cancer cells. British Journal of Cancer, 118(2), 200-212.

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Culturing Human IGR39 Melanoma Cells

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Human IGR39 melanoma cells were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH. Cells were cultured in growth medium (GM): DMEM (Dulbecco's Modified Eagle's Medium (ECB7501L, EuroClone, Italy) supplemented with 10% fetal bovine serum (FBS, ECS0180D, Eu-roClone, Italy), 1% non-essential amminoacids (NEA, ECB3054D, EuroClone, Italy), 1% antibiotics (Penicillin/Streptomycin, Pen/Strep, ECB3001D, EuroClone, Italy), 1% L-Glutamine (L-Glut, ECB3000D-20, EuroClone, Italy) at 37 • C and 5% CO 2 and 95% humidity.

Lionetti M.C., Fumagalli M.R, & La Porta C.A.M. (2024). Nuclear Biophysical Changes during Human Melanoma Plasticity. Cells, tissues, organs, 213(2).

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