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C maf percp efluor symof1 710

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The C-Maf PerCP-eFluor (symOF1) 710 is a fluorescently labeled antibody specific for the transcription factor c-Maf. It is designed for use in flow cytometry applications to detect and quantify c-Maf-expressing cells.

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Lab products found in correlation

Permeabilization buffer, by Thermo Fisher Scientific (2 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti il 2 jes 5h4 apc cy7, by BD (2 mentions) Anti ifn γ pe cy7 xmg1.2, by BD (2 mentions) Anti il 10 jes5 16e3 apc, by Thermo Fisher Scientific (2 mentions) Anti il17a ebi17b7 fitc, by Thermo Fisher Scientific (2 mentions) Igg2bk isotype bmg2b efluor 710 control, by Thermo Fisher Scientific (2 mentions) Brefeldin a, by Merck Group (2 mentions)

Spelling variants of this product

PerCP-eFluor 710 (33 citations) C-Maf (2 citations) SymOF1; (2 citations)

2 protocols using c maf percp efluor symof1 710

1

T Cell Stimulation and Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
After differentiation, T cells were re-stimulated with anti-CD3 and anti-CD28 at 2 μg/ml each or PDBU (Sigma-Aldrich) and Ionomycin (Calbiochem) at 500 ng/ml each (TH17) for 4 hours, with Brefeldin A (10 μg/ml, Sigma-Aldrich) added for the last 2 and 4 hours of culture, respectively. Surface markers were stained in PBS together with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Molecular Probes). For intracellular cytokine detection, cells were fixed with 2% formaldehyde, permeabilized with permeabilization buffer (eBioscience) and stained with: anti-IL-2 (JES-5H4) APC-Cy7 (BD), anti-IFN-γ (XMG1.2) PE-Cy7 (BD) and anti-IL-4 (11B11) PE, anti-IL-10 (JES5-16E3) APC, anti-IL17A (eBi17B7) FITC (eBioscience). For Foxp3 and c-Maf staining, cells were fixed with Fixation/permeabilization buffers and stained with Foxp3 (FJK-16S) PE or c-Maf PerCP-eFluor (symOF1) 710 with IgG2bK isotype (BMG2b) eFluor 710 control (eBioscience).
Gabryšová L., Alvarez-Martinez M., Luisier R., Cox L.S., Sodenkamp J., Hosking C., Pérez-Mazliah D., Whicher C., Kannan Y., Potempa K., Wu X., Bhaw L., Wende H., Sieweke M.H., Elgar G., Wilson M., Briscoe J., Metzis V., Langhorne J., Luscombe N.M, & O’Garra A. (2018). c-Maf controls immune responses by regulating disease-specific gene networks and repressing IL-2 in CD4+ T cells. Nature immunology, 19(5), 497-507.
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2

T Cell Stimulation and Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
After differentiation, T cells were re-stimulated with anti-CD3 and anti-CD28 at 2 μg/ml each or PDBU (Sigma-Aldrich) and Ionomycin (Calbiochem) at 500 ng/ml each (TH17) for 4 hours, with Brefeldin A (10 μg/ml, Sigma-Aldrich) added for the last 2 and 4 hours of culture, respectively. Surface markers were stained in PBS together with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Molecular Probes). For intracellular cytokine detection, cells were fixed with 2% formaldehyde, permeabilized with permeabilization buffer (eBioscience) and stained with: anti-IL-2 (JES-5H4) APC-Cy7 (BD), anti-IFN-γ (XMG1.2) PE-Cy7 (BD) and anti-IL-4 (11B11) PE, anti-IL-10 (JES5-16E3) APC, anti-IL17A (eBi17B7) FITC (eBioscience). For Foxp3 and c-Maf staining, cells were fixed with Fixation/permeabilization buffers and stained with Foxp3 (FJK-16S) PE or c-Maf PerCP-eFluor (symOF1) 710 with IgG2bK isotype (BMG2b) eFluor 710 control (eBioscience).
Gabryšová L., Alvarez-Martinez M., Luisier R., Cox L.S., Sodenkamp J., Hosking C., Pérez-Mazliah D., Whicher C., Kannan Y., Potempa K., Wu X., Bhaw L., Wende H., Sieweke M.H., Elgar G., Wilson M., Briscoe J., Metzis V., Langhorne J., Luscombe N.M, & O’Garra A. (2018). c-Maf controls immune responses by regulating disease-specific gene networks and repressing IL-2 in CD4+ T cells. Nature immunology, 19(5), 497-507.
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