Triglyceride mix

Manufactured by Merck Group

Sourced in United States

The Triglyceride mix is a laboratory standard used for the calibration and quality control of analytical instruments that measure triglyceride levels. It contains a known concentration of triglycerides and is designed to provide a consistent reference point for accurate measurement of triglyceride values in samples.

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Lab products found in correlation

Bilirubin, by Merck Group (2 mentions) Hemoglobin, by Merck Group (2 mentions) Loperamide, by Johnson & Johnson (2 mentions) Acetaminophen, by Johnson & Johnson (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions) Fluvastatin, by Merck Group (1 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) Blank human plasma, by Equitech-Bio (1 mentions) Uv 2550, by Shimadzu (1 mentions)

5 protocols using triglyceride mix

Interference Testing for Analytical Assay

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Interference tests were performed with the following substances: human blood, barium sulfate (contrast medium), hemoglobin (Sigma Aldrich Co., St. Louis, MO, USA), bilirubin (Sigma Aldrich Co.), triglyceride mix (Sigma Aldrich Co.), loperamide (anti-diarrhea drug; Janssen, Seoul, Korea), metronidazole (antibiotic; CJ Pharma, Seoul, Korea), cephradine (SCD Pharm, Seoul, Korea), cefuroxime (SCD Pharm), cefpodoxime (antibiotic; CJ Pharma), cefixime (Hanmi Pharm, Seoul, Korea), tetracycline (Chong Kun Dang Pharm, Seoul, Korea), levofloxacin (Jeil Pharm, Seoul, Korea), amoxicillin (Chong Kun Dang Pharm), ibuprofen (Samil Pharm, Yeosu, Korea), and acetaminophen (Janssen).

Kim J.S., Lee S.K., Ko D.H., Hyun J, & Kim H.S. (2018). Performance Evaluation of the Automated Fluorescent Immunoassay System Rotavirus Assay in Clinical Samples. Annals of Laboratory Medicine, 39(1), 50-57.

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Interference Testing of Clinical Assays

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Interference tests were performed with the following substances: human blood, barium sulfate (contrast medium), loperamide (anti-diarrhea drug, Janssen, Seoul, Korea), metronidazole (antibiotics, CJ Pharma, Seoul, Korea), hemoglobin (Sigma-Aldrich Co., St. Louis, MO, USA), bilirubin (Sigma-Aldrich Co.), and triglyceride mix (Sigma-Aldrich Co.). Each substance (5 mg) was dissolved in 1 mL of solvent (hemoglobin in distilled water; triglyceride in ether; bilirubin in chloroform; all others in distilled water), and 50 µL of solution was mixed with both 950 µL of negative stool suspension (negative base pool) and 950 µL of low positive stool suspension (low positive base pool). The final concentrations of barium sulfate, loperamide, metronidazole, hemoglobin, bilirubin, and triglyceride mix for interference testing were 0.25 mg/mL each. For substances in the liquid form (e.g., blood), 50 µL of the substance was mixed with 950 µL of the negative and low positive base pool (1:20 dilution).

Kim J., Kim H.S., Kim H.S., Kim J.S., Song W., Lee K.M., Lee S., Park K.U., Lee W, & Hong Y.J. (2014). Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples. Annals of Laboratory Medicine, 34(3), 216-222.

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Lipidomic Analysis of Diverse Lipid Standards

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Phosphatidylcholine preparation from chicken egg (840051P, Avanti Polar Lipids), Escherichia coli polar lipid extract (100600P, Avanti Polar Lipids), phosphatidyl serines from porcine brain (840032P, Avanti Polar Lipids), ceramide from porcine brain (860052P, Avanti Polar Lipids) and ceramide from chicken egg (860051P, Avanti Polar Lipids) were obtained from Avanti Polar Lipids (Otto Nordwald GmbH, Germany) and dissolved in MeOH at a concentration of 1 mg/mL. Additionally, L-alpha-Phosphatidylinositol sodium salt from Glycine max (P0639), Triglyceride mix (17811-AMP), 1,3-Dioleoyl-2-palmitioyl-glycerol (D1657), Glyceryl tritricosanoate (T1412), Glyceryl trioleate (T7140) and 1,2-Dilinoleoy-3-palmitoyl-rac-glycerol (D0301) were obtained from Sigma-Aldrich (Taufkirchen, Germany) and dissolved in either MeOH, MTBE, CHCl3 or solvent mixtures, depending on solubility. Different samples for analysis were prepared and diluted in ACN/iPrOH/water (65/30/5, v/v/v) to 10 μg/mL for analysis. The following lipid classes and standard samples were analysed and are named throughout the paper as indicated in brackets: Phophatidylcholines (PC, LMGP0101), phosphatidylethanolamines (PE, LMGP0201), phosphatidylglycerols (PG, LMGP04011), phosphatidylserines (PS, LMGP0301), phosphatidylinositols (PI, LMGP0601), ceramides (Cer, LMSP0201/ LMSP0202), and triacylglycerols (TG, LMGL0301).

Witting M., Ruttkies C., Neumann S, & Schmitt-Kopplin P. (2017). LipidFrag: Improving reliability of in silico fragmentation of lipids and application to the Caenorhabditis elegans lipidome. PLoS ONE, 12(3), e0172311.

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Quantification of statins and metabolites

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SMV, ATV, fluvastatin, triglyceride mix, and LC-MS grade acetonitrile, water, methanol, ammonium formate (≥99.0%), and formic acid (~98%) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). RSV, SMV-A, 2-OH-ATV, and 4-OH-ATV were purchased from Toronto Research Chemicals Inc. (Toronto, Ontario, Canada). Blank human plasma was purchased from Equitech-Bio, Inc. (Kerrville, TX, USA). Ethyl acetate (spectrometric grade, 99.5+%) was purchased from Alfa Aesar (Ward Hill, MA, USA).

El-Zailik A., Cheung L.K., Wang Y., Sherman V, & Chow D.S. (2018). Simultaneous LC-MS/MS analysis of simvastatin, atorvastatin, rosuvastatin and their active metabolites for plasma samples of obese patients underwent gastric bypass surgery. Journal of pharmaceutical and biomedical analysis, 164, 258-267.

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Quantifying Singlet Oxygen Generation

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The amount of ¹O₂ was measured using 9,10-diphenylanthracene, which specifically reacts with ¹O₂ to form an endoperoxide accompanied by a decrease in absorbance at 355 nm, indicating the production of ¹O₂47 (link). Briefly, HA was dissolved in petroleum ether, liquid paraffin and n-heptane (3:1), n-heptane, dichloromethane, chloroform or acetone, at a final concentration of 4 μg/ml and separated into two groups. Triglyceride mix (Sigma-Aldrich, catalogue No. 17811-1AMP) was added to the chloroform or acetone at a final concentration of 10 mg/ml. The addition of 20 μg/ml 9,10-diphenylanthracene was used to trap the ¹O₂ produced by HA. One group was irradiated by light for 30 min, and the other group was maintained in darkness. Then, the absorbance of each sample was measured at 355 nm using an ultraviolet–visible spectrophotometer (UV-2550, Shimadzu Japan). The decreased percentage was calculated to indicate the production of ¹O₂ generated by HA.

Chang W., Zhang M., Zheng S., Li Y., Li X., Li W., Li G., Lin Z., Xie Z., Zhao Z, & Lou H. (2015). Trapping toxins within lipid droplets is a resistance mechanism in fungi. Scientific Reports, 5, 15133.

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