PBMCs were harvested from healthy donor’s blood and isolated by Ficoll gradients. Fresh anticoagulated whole blood was added onto the half volume of Ficoll-Paque PLUS (Sigma-Aldrich) in a centrifuge tube, and centrifuged at 1500 rpm for 40 min. The PBMC interface layer was gently collected and washed twice with filter-sterilized PBS by centrifugation at 1000 rpm for 10 min. Next, CD3+ T cells were separated from freshly PBMCs by using a MojoSort Human CD3+ T-cell Isolation Kit (Biolegend, United States), cultured in CD3-coated plates (clone OKT3; Biolegend), and then cultured in medium (RPMI1640 with 10% FBS, penicillin/streptomycin, and 2 ng/ml IL-2).
Cd3 coated plates clone okt3
Manufactured by BioLegend
Sourced in United States
CD3-coated plates (clone OKT3) are a type of cell culture plate designed for the activation of T cells. The plates are coated with the anti-CD3 monoclonal antibody clone OKT3, which binds to the CD3 complex on the surface of T cells, providing a signal for T cell activation.
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Lab products found in correlation
2 protocols using cd3 coated plates clone okt3
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Isolation and Culture of Human CD3+ T Cells
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Isolation and Culture of Human CD3+ T Cells
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Peripheral blood from normal healthy donors was collected into EDTA potassium vacuoles.
PBMCs were isolated using Ficoll lymphocyte isolation medium. Next, CD3 + T lymphocytes were purified (> 98%) by negative selection with MojoSort TM Human CD3 + T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3; Biolegend) and cultured in medium (RPMI1640 with 10 % FBS, penicillin/streptomycin, and 2 ng mL -1 IL-2).
PBMCs were isolated using Ficoll lymphocyte isolation medium. Next, CD3 + T lymphocytes were purified (> 98%) by negative selection with MojoSort TM Human CD3 + T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3; Biolegend) and cultured in medium (RPMI1640 with 10 % FBS, penicillin/streptomycin, and 2 ng mL -1 IL-2).
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