Apopnexin annexin 5 fitc apoptosis detection kit

Manufactured by Merck Group

Sourced in United States

The ApopNexin Annexin V FITC Apoptosis detection kit is a laboratory assay used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. The Annexin V is conjugated with the fluorescent dye FITC, allowing for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Facsort flow cytometer, by BD (1 mentions) Caspase glo 3 7 reagent, by Promega (1 mentions)

Spelling variants of this product

Annexin V-FITC Apoptosis Detection Kit (446 citations) Annexin V-FITC (217 citations) Annexin V-FITC kit (29 citations) Annexin V-FITC apoptosis kit (21 citations) ApopNexin™ FITC Apoptosis Detection Kit (19 citations) Annexin V Apoptosis Detection Kit (13 citations) ApopNexin Annexin V FITC Apoptosis kit (7 citations) Annexin V-FITC detection kit (4 citations) Annexin V-FITC Apoptosis (2 citations) Annexin V Apoptosis Kit (2 citations)

2 protocols using apopnexin annexin 5 fitc apoptosis detection kit

Apoptosis Analysis in Rat Liver and Cells

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The extent of apoptosis in the livers of control and tributyrin-treated rats was analyzed for the presence of apoptotic bodies as described previously [12 (link)]. Apoptosis in the rat HCC JM1 cells was evaluated by annexin-V/propidium iodide staining and caspases 3/7 activity assays. Cells were stained using an ApopNexin Annexin V FITC Apoptosis detection kit (EMD Millipore, Billerica, MA), according to the manufacturer's protocol and analyzed using a FACSort flow cytometer (Becton Dickinson, Washington, DC). Heat-shock treated cells were used as a positive control, and non-stained cells as a negative control. Quantitative analyses of caspases-3/7 activity in cells were conducted by ELISA, using Caspase-Glo^®3/7 reagent (Promega, Madison, USA) as substrate, according to manufacturer's instructions.

Ortega J.F., de Conti A., Tryndyak V., Furtado K.S., Heidor R., Horst M.A., Fernandes L.H., Tavares P.E., Pogribna M., Shpyleva S., Beland F.A., Pogribny I.P, & Moreno F.S. (2016). Suppressing activity of tributyrin on hepatocarcinogenesis is associated with inhibiting the p53-CRM1 interaction and changing the cellular compartmentalization of p53 protein. Oncotarget, 7(17), 24339-24347.

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Apoptosis Quantification in HCECs

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The percentage of HCECs undergoing apoptosis was investigated by using a detection kit (apopNexin Annexin V-FITC Apoptosis Detection Kit; EMD Millipore, Lake Placid, NY, USA). The HCECs were trypsinized and incubated with annexin V and propidium iodide (PI) for 15 minutes and analyzed by flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) to determine the percentage of apoptotic cells in each sample.

Li Y., Ma X., Li J., Yang L., Zhao X., Qi X., Zhang X., Zhou Q, & Shi W. (2019). Corneal Denervation Causes Epithelial Apoptosis Through Inhibiting NAD+ Biosynthesis. Investigative ophthalmology & visual science, 60(10).

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