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Alexa fluor 488 anti brdu

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Alexa Fluor 488 anti-BrdU is a fluorescent antibody used to detect 5-bromo-2'-deoxyuridine (BrdU) incorporation in cells. It is conjugated to the Alexa Fluor 488 dye, which emits green fluorescence when excited.

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Lab products found in correlation

Apo brdu tunel assay kit, by Thermo Fisher Scientific (2 mentions) Live dead fixable for far red dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Facscalibur, by BD (2 mentions)

2 protocols using alexa fluor 488 anti brdu

1

Apoptosis Analysis by FACS

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Cells with and without serum starvation for 24 h were fixed and permeabilized. Cells were then stained with APO-BrdU TUNEL Assay Kit with Alexa Fluor 488 anti-Brdu (A23210, ThermoFisher Scientific) and LIVE/DEAD Fixable for Far Red Dead Cell Stain Kit, for 633 nm excitation (L34973, ThermoFisher Scientific). Apoptosis was assessed by FACS analysis using FACSCalibur (Becton Dickinson) under 488 nm and 635 nm lasers. Cells negative for both far-red dye and TUNEL staining are live cells; far-red-dye negative, TUNEL-positive staining cells are early apoptotic cells; far-red-dye positive, TUNEL-negative staining cells are dead cells; far-red-dye positive, TUNEL-positive staining cells are primarily cells in late stages of apoptosis. The total percentage of early and late apoptotic cells were compared.
Tang J., Zhou H., Sahay K., Xu W., Yang J., Zhang W, & Chen W. (2018). Obesity-associated family with sequence similarity 13, member A (FAM13A) is dispensable for adipose development and insulin sensitivity. International Journal of Obesity (2005), 43(6), 1269-1280.
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2

Apoptosis Quantification by Flow Cytometry

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Cells with and without serum starvation for 24 h were fixed and permeabilized. Cells were then stained with APO-BrdU TUNEL Assay Kit with Alexa Fluor 488 anti-Brdu (A23210, ThermoFisher Scientific) and LIVE/DEAD Fixable for Far Red Dead Cell Stain Kit, for 633 nm excitation (L34973, ThermoFisher Scientific). Apoptosis was assessed by FACS analysis using FACSCalibur (Becton Dickinson) under 488 nm and 635 nm lasers. Cells negative for both far-red dye and TUNEL staining are live cells; far-red-dye negative, TUNEL-positive staining cells are early apoptotic cells; far-red-dye positive, TUNEL-negative staining cells are dead cells; far-red-dye positive, TUNEL-positive staining cells are primarily cells in late stages of apoptosis. The total percentage of early and late apoptotic cells were compared.
Tang J., Zhou H., Sahay K., Xu W., Yang J., Zhang W, & Chen W. (2018). Obesity-associated family with sequence similarity 13, member A (FAM13A) is dispensable for adipose development and insulin sensitivity. International Journal of Obesity (2005), 43(6), 1269-1280.
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