100 μL of freshly filtered-collected aqueous humor samples were collected in the new tube, and the exosome was isolated using ExoQuick-TC Ultra for Tissue Culture Media (System Bioscience, #EQULTRA-20TC-1, US), modifying the manufacturer’s protocol to accommodate the smaller sample volume. Briefly, the buffers were used for 1/20 volume than the recommended protocol. The isolated exosomes were ultracentrifuged for 120000 × g, 4 °C for 2 h, and the supernatant was discarded without disturbing the pellet. Pellets were reconstituted or lysed in buffers for the next analyses.
Exoquick tc ultra for tissue culture media
Manufactured by System Biosciences
Sourced in United States
ExoQuick-TC Ultra for Tissue Culture Media is a proprietary reagent developed by System Biosciences for the isolation and concentration of extracellular vesicles (EVs) from tissue culture media. The product is designed to facilitate the rapid and efficient recovery of EVs from cell culture supernatants.
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Lab products found in correlation
2 protocols using exoquick tc ultra for tissue culture media
1
Exosome Isolation from Aqueous Humor
2
Exosome Isolation from Conditioned Media
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ExoQuick-TC kit (ExoQuick-TC Ultra for Tissue Culture Media, System Biosciences, Cat. #: EQULTEA-20TC-1) was used as per manufacturer’s recommendation. Specifically, 5 mL of CM must be used for EV isolation using the kit. Therefore, 2 mL of basic culture medium (RPMI-1640 for CM from EBC1 and H596 cells; DMEM for CM from Hs746T cells) was added to CM concentrates that were obtained following TFF (as described above) in order to bring the total volume of CM to 5 mL. No other changes were made to the process recommended by the manufacturer of ExoQuick-TC.
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