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Cd38 alexa fluor 680

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CD38-Alexa Fluor 680 is a fluorescently labeled antibody that targets the CD38 protein. It is designed for use in flow cytometry and other immunoassays.

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Lab products found in correlation

Cd8 bv510, by BioLegend (2 mentions) Cd14 bv510, by BioLegend (2 mentions) Facsaria 2, by BD (2 mentions) Live dead aqua, by Thermo Fisher Scientific (2 mentions) Cd56 bv510, by BioLegend (2 mentions) Cd21 cy5pe, by BD (2 mentions) Igd cy7pe, by BD (2 mentions) Igm cy5.5 percp, by BD (2 mentions) Igg cy7pe, by BD (2 mentions) Cd27 qd605, by Thermo Fisher Scientific (2 mentions) Cd19 ecd, by Beckman Coulter (2 mentions) Anti cd3 bv510, by BioLegend (2 mentions)

2 protocols using cd38 alexa fluor 680

1

PfCSP-reactive Memory B Cell Isolation

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PBMCs (106) were stained for viability using the amine-reactive dye Aqua LIVE/DEAD (Invitrogen) followed by staining for surface markers. In addition to the tetramer probes rPfCSP-APC, (NANP)9-PE generated above, the staining panels included: anti-CD3-BV510 (BioLegend), CD8-BV510 (BioLegend), CD14-BV510 (BioLegend), CD56-BV510 (BioLegend), CD19-ECD (Beckman), CD27-QD605 (Invitrogen), CD21-Cy5PE (Becton Dickenson), CD38-Alexa Fluor 680 (Becton Dickenson), IgD-Cy7PE (Becton Dickenson), IgM-Cy5.5-PerCP (Becton Dickenson), and IgG- Cy7PE (Becton Dickenson). Cells were acquired and sorted using a BD FACS Aria II instrument (BD Immunocytometry Systems), and fluorescence-activated cell sorting (FACS) data was analyzed using FlowJo software (Tree Star). Gating strategy is shown in Fig. 1a. PfCSP-reactive (rPfCSP+ and/or (NANP)9+) CD19+CD27+IgG+ IgD−IgM− memory B cells were single cell, dry-sorted into 96-well PCR plates, rapidly frozen on dry ice and stored at −80°C until processing41 (link),42 (link).
Kisalu N.K., Idris A.H., Weidle C., Flores-Garcia Y., Flynn B.J., Sack B.K., Murphy S., Schön A., Freire E., Francica J.R., Miller A.B., Gregory J., March S., Liao H.X., Haynes B.F., Wiehe K., Trama A.M., Saunders K.O., Gladden M.A., Monroe A., Bonsignori M., Kanekiyo M., Wheatley A.K., McDermott A.B., Farney S.K., Chuang G.Y., Zhang B., Natasha K.C., Chakravarty S., Kwong P.D., Sinnis P., Bhatia S.N., Kappe S.H., Sim B.K., Hoffman S.L., Zavala F., Pancera M, & Seder R.A. (2018). A human monoclonal antibody prevents malaria infection and defines a new site of vulnerability on Plasmodium falciparum circumsporozoite protein. Nature medicine, 24(4), 408-416.
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2

PfCSP-reactive Memory B Cell Isolation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs (106) were stained for viability using the amine-reactive dye Aqua LIVE/DEAD (Invitrogen) followed by staining for surface markers. In addition to the tetramer probes rPfCSP-APC, (NANP)9-PE generated above, the staining panels included: anti-CD3-BV510 (BioLegend), CD8-BV510 (BioLegend), CD14-BV510 (BioLegend), CD56-BV510 (BioLegend), CD19-ECD (Beckman), CD27-QD605 (Invitrogen), CD21-Cy5PE (Becton Dickenson), CD38-Alexa Fluor 680 (Becton Dickenson), IgD-Cy7PE (Becton Dickenson), IgM-Cy5.5-PerCP (Becton Dickenson), and IgG- Cy7PE (Becton Dickenson). Cells were acquired and sorted using a BD FACS Aria II instrument (BD Immunocytometry Systems), and fluorescence-activated cell sorting (FACS) data was analyzed using FlowJo software (Tree Star). Gating strategy is shown in Fig. 1a. PfCSP-reactive (rPfCSP+ and/or (NANP)9+) CD19+CD27+IgG+ IgD−IgM− memory B cells were single cell, dry-sorted into 96-well PCR plates, rapidly frozen on dry ice and stored at −80°C until processing41 (link),42 (link).
Kisalu N.K., Idris A.H., Weidle C., Flores-Garcia Y., Flynn B.J., Sack B.K., Murphy S., Schön A., Freire E., Francica J.R., Miller A.B., Gregory J., March S., Liao H.X., Haynes B.F., Wiehe K., Trama A.M., Saunders K.O., Gladden M.A., Monroe A., Bonsignori M., Kanekiyo M., Wheatley A.K., McDermott A.B., Farney S.K., Chuang G.Y., Zhang B., Natasha K.C., Chakravarty S., Kwong P.D., Sinnis P., Bhatia S.N., Kappe S.H., Sim B.K., Hoffman S.L., Zavala F., Pancera M, & Seder R.A. (2018). A human monoclonal antibody prevents malaria infection and defines a new site of vulnerability on Plasmodium falciparum circumsporozoite protein. Nature medicine, 24(4), 408-416.
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