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Spe100

Manufactured by Leica
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Sourced in Germany

The SPE100 is a high-performance spectrometer designed for laboratory applications. It features a compact and robust design, with a wavelength range of 200-1100 nm and a resolution of up to 0.1 nm. The SPE100 provides accurate and reliable spectral measurements, making it a versatile tool for various analytical tasks.

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Lab products found in correlation

A11008, by Thermo Fisher Scientific (2 mentions) A 11030, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) A11081, by Thermo Fisher Scientific (2 mentions) Ab6640, by Abcam (2 mentions) A 11056, by Thermo Fisher Scientific (2 mentions) Ab82527, by Abcam (2 mentions) Ab84593, by Abcam (2 mentions) Ab102010, by Abcam (2 mentions) Sc 30820, by Santa Cruz Biotechnology (2 mentions)

2 protocols using spe100

1

Histological and Immunohistochemical Analysis of Testis and Epididymis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were harvested at necropsy and fixed overnight in 4% paraformaldehyde (PFA) in PBS. Subsequently, 5 μm-thick testis and epididymal sections from infected and uninfected mice were processed for histology by H & E staining. For immunohistochemistry, the tissue sections were incubated with mouse primary monoclonal anti-CD45 (610266; BD Biosciences), anti-ETV5 (ab102010; Abcam), and anti-GATA4 (ab84593; Abcam), rabbit polyclonal anti-Lin28a (3978S, Cell Signaling), rat polyclonal anti-TRA98 (ab82527; Abcam), rat polyclonal anti-F4/80 (ab6640; Abcam), or goat polyclonal anti-3β-HSD antibodies (SC-30820, Santa Cruz Biotechnology). After washing, slides were stained with Alexa Fluor 488- or, Alexa Fluor 546-conjugated goat anti-rabbit, goat anti-mouse, or donkey anti-goat (1:1000; A11008, A11081, A11030, or A11056; ThermoFisher Scientific) secondary antibodies for 1 h, and mounted with prolong gold anti-fade mount containing the nuclear counter stain, DAPI (ThermoFisher Scientific). Immunostaining was detected by confocal microscopy (Leica SPE100, Germany).
Govero J., Esakky P., Scheaffer S.M., Fernandez E., Drury A., Platt D.J., Gorman M.J., Richner J.M., Caine E.A., Salazar V., Moley K.H, & Diamond M.S. (2016). Zika virus infection damages the testes in mice. Nature, 540(7633), 438-442.
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2

Histological and Immunohistochemical Analysis of Testis and Epididymis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were harvested at necropsy and fixed overnight in 4% paraformaldehyde (PFA) in PBS. Subsequently, 5 μm-thick testis and epididymal sections from infected and uninfected mice were processed for histology by H & E staining. For immunohistochemistry, the tissue sections were incubated with mouse primary monoclonal anti-CD45 (610266; BD Biosciences), anti-ETV5 (ab102010; Abcam), and anti-GATA4 (ab84593; Abcam), rabbit polyclonal anti-Lin28a (3978S, Cell Signaling), rat polyclonal anti-TRA98 (ab82527; Abcam), rat polyclonal anti-F4/80 (ab6640; Abcam), or goat polyclonal anti-3β-HSD antibodies (SC-30820, Santa Cruz Biotechnology). After washing, slides were stained with Alexa Fluor 488- or, Alexa Fluor 546-conjugated goat anti-rabbit, goat anti-mouse, or donkey anti-goat (1:1000; A11008, A11081, A11030, or A11056; ThermoFisher Scientific) secondary antibodies for 1 h, and mounted with prolong gold anti-fade mount containing the nuclear counter stain, DAPI (ThermoFisher Scientific). Immunostaining was detected by confocal microscopy (Leica SPE100, Germany).
Govero J., Esakky P., Scheaffer S.M., Fernandez E., Drury A., Platt D.J., Gorman M.J., Richner J.M., Caine E.A., Salazar V., Moley K.H, & Diamond M.S. (2016). Zika virus infection damages the testes in mice. Nature, 540(7633), 438-442.
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