The reagent 1-ethyl-3-[3-dimethylaminopropil]carbodiimide (EDAC) was purchased from Alfa Aesar (Karlsruhe, Germany). Ethylenediamine (EDA), sodium borohydride, sodium periodate, 2,4,6-trinitrobenzenesulfonic (TNBS) acid solution, Schiff’s reagent, all dextran polymers (dextran sizes ranged from 1.5 kDa to 40 kDa), p-nitrophenyl butyrate (pNPB), epichlorohydrin, dithiothreitol (DTT) and d ,l -cysteine were acquired from Sigma (St. Louis, MO, USA). 6-Nitro-3-phenylacetamidobenzoic acid (NIPAB) was purchased from Fluorochem (Hadfield, UK). Octyl–Sepharose 4BCL (OA) was purchased from GE Healthcare (Uppsala, Sweden). Toyopearl HW65C was purchased from Tosoh Bioscience GmbH. (Griesheim, Germany). The enzymes Candida antarctica lipase B (CALB), Thermomyces lanuginosus lipase (TLL), and Rhizomucor miehei lipase (RML) were kindly donated by Novozymes (Bagsvard, Denmark). Penicillin G acylase (PGA) was kindly donated by Antibioticos S.A. (León, Spain). All other reagents were analytical or HPLC grade. Enzymatic assays were carried out on a V-730 spectrophotometer from JASCO Analitica Spain S.L. (Madrid, Spain).
Rhizomucor miehei lipase
Manufactured by Novozymes
Sourced in Denmark
Rhizomucor miehei lipase is a microbial enzyme derived from the fungus Rhizomucor miehei. It is a type of lipase, an enzyme that catalyzes the hydrolysis of fats and oils. The core function of this lipase is to facilitate the breakdown of lipid substrates.
Automatically generated - may contain errors
Lab products found in correlation
Dithiothreitol (dtt), by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Sodium periodate, by Merck Group (1 mentions)
Schiff s reagent, by Merck Group (1 mentions)
Candida antarctica lipase b, by Novozymes (1 mentions)
2 4 6 trinitrobenzenesulfonic acid solution tnbs, by Merck Group (1 mentions)
Dl cysteine, by Merck Group (1 mentions)
Thermomyces lanuginosus lipase, by Novozymes (1 mentions)
Lipase from aspergillus niger, by Merck Group (1 mentions)
Rhizopus oryzae lipase, by Merck Group (1 mentions)
Pseudomonas fluorescens lipase, by Merck Group (1 mentions)
Candida antarctica lipase type b, by Merck Group (1 mentions)
Esterase, by Merck Group (1 mentions)
Porcine pancreas lipase, by Merck Group (1 mentions)
Amano lipase, by Merck Group (1 mentions)
Thermomyces lanuginosus lipase, by Merck Group (1 mentions)
Candida rugosa lipase, by Merck Group (1 mentions)
2 protocols using rhizomucor miehei lipase
1
Immobilization of Enzymatic Catalysts
2
Comparative Evaluation of Lipase Preparations
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Commercially available lipases were used as follows: (i) Immobilized lipases:
Candida antarctica lipase type B immobilized on acrylic resin (CAL-B, 7,300.0 U/g), Thermomyces lanuginosus lipase immobilized on immobead-150 (TLL, 250.0 U/g), Rhizopus oryzae lipase immobilized on immobead-150 (ROL, 340.0 U/g), Pseudomonas fluorescens lipase immobilized on immobead-150 (PFL, ≥ 600 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel (AK, ≥ 30 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel on pumice (AK, ≥ 8.0 U/g) and Amano lipase PS from Burkholderia cepacia immobilized on diatomaceous earth (PS-IM, ≥ 500 U/g) were acquired from Sigma-Aldrich®, Rhizomucor miehei lipase immobilized in anionic resin (RML, 150.0 U/g) was purchased from Novozymes ® . (ii) Unsupported lipase preparations: Pseudomonas fluorescens lipase (AK, 22,100.0 U/g), Penicillium camemberti lipase (G, 50.0 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), Amano lipase from Mucor javanicus (MJL, ≥ 10,000 U/g) and lipase from Aspergillus niger (ANL, 200 U/g) were acquired from Sigma-Aldrich ® . Porcine pancreas lipase (PPL, 46.0 U/g solid), and Candida rugosa lipase (CRL, 1.4 U/g) were obtained from Sigma-Aldrich ® . (iii) Esterase, immobilized in Eupergit ® C from hog liver (~200 U/g) was obtained from Sigma-Aldrich ® .
Candida antarctica lipase type B immobilized on acrylic resin (CAL-B, 7,300.0 U/g), Thermomyces lanuginosus lipase immobilized on immobead-150 (TLL, 250.0 U/g), Rhizopus oryzae lipase immobilized on immobead-150 (ROL, 340.0 U/g), Pseudomonas fluorescens lipase immobilized on immobead-150 (PFL, ≥ 600 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel (AK, ≥ 30 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel on pumice (AK, ≥ 8.0 U/g) and Amano lipase PS from Burkholderia cepacia immobilized on diatomaceous earth (PS-IM, ≥ 500 U/g) were acquired from Sigma-Aldrich®, Rhizomucor miehei lipase immobilized in anionic resin (RML, 150.0 U/g) was purchased from Novozymes ® . (ii) Unsupported lipase preparations: Pseudomonas fluorescens lipase (AK, 22,100.0 U/g), Penicillium camemberti lipase (G, 50.0 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), Amano lipase from Mucor javanicus (MJL, ≥ 10,000 U/g) and lipase from Aspergillus niger (ANL, 200 U/g) were acquired from Sigma-Aldrich ® . Porcine pancreas lipase (PPL, 46.0 U/g solid), and Candida rugosa lipase (CRL, 1.4 U/g) were obtained from Sigma-Aldrich ® . (iii) Esterase, immobilized in Eupergit ® C from hog liver (~200 U/g) was obtained from Sigma-Aldrich ® .
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