Hdl cholesterol

Primary antibody specific for lacteal marker lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1; #67538), p120-catenin (#59854), and Vascular cell adhesion molecule 1 (VCAM1; #39036) from cell signaling (Danvers, MA, USA), Zonula occludens-1 (ZO1; #sc-33725) from Santa Cruz Biotechnology (Dallas, TX, USA), Plasmalemma vesicle-associated protein 1 (PLVAP1; #NB100-77668), and ATP-binding cassette subfamily G member 1 (ABCG1; #NB400-132) from Novus (Centennial, CO) were purchased. The ELISA assay kits for LDL-cholesterol (#79980) and HDL-cholesterol (#79990) were purchased from Crystal Chem (Elk Grove Village, IL, USA). Triglyceride assay (#10010303) and Free Fatty Acid assay (#STA-618) kits were purchased from Cayman chemicals (Ann Arbor, MI, USA) and Cell Biolabs, Inc. (San Diego, CA, USA), respectively.

Plasma glucose was determined using the glucose oxidase method. Plasma NEFA (Wako Chemicals, Richmond, VA, USA), triacylglycerols (Roche Diagnostics, Indianapolis, IN, USA), and total cholesterol (Wako Chemicals) and HDL-cholesterol (Crystal Chem, Elk Grove Village, IL, USA) were determined using enzymatic assays. Plasma insulin (ALPCO, Salem, NH, USA) and C-peptide (Millipore, Burlington, MA, USA) were determined by ELISA. GC-MS was used to determine plasma [6,6-²H]glucose enrichment [27 (link)]. Plasma amino acid analysis was performed as previously described [28 (link)].