All procedures were performed in accordance with protocols approved by the Johns Hopkins University Animal Care and Use Committee. Twelve mice (8 male, 4 female) were obtained by crossing PV cre lines 83 (Jackson Labs: 008069) with Ai32 lines 84 (Jackson Labs: 012569). Seven PV cre ; Ai32 mice (5 male, 2 female) were trained to perform the cross-modal selection task and included in behavioral and optogenetic inhibition experiments. Five PV cre ; Ai32 mice (3 male, 2 female) were trained to perform the tactile detection task and included in optogenetic inhibition experiments. Four male mice included in behavioral experiments were obtained by crossing Emx1 cre mice 85 (Jackson Labs: 005628) with Ai32 mice. Two male mice included in behavioral experiments were heterozygous VGAT ChR2-EYFP (Jackson Labs: 014548) 86 . Mice ranged in age from 2-5 months at the start of training. Mice were housed in a vivarium with a reverse light-dark cycle (12 h each phase), and were singly housed after surgery and during behavioral experiments. Details of assignment to different experimental conditions are listed in Table 1 .
Vgat chr2 eyfp
Manufactured by Jackson ImmunoResearch
VGAT-ChR2-EYFP is a genetic construct that expresses the light-sensitive protein channelrhodopsin-2 (ChR2) and the enhanced yellow fluorescent protein (EYFP) under the control of the vesicular GABA transporter (VGAT) promoter. This construct allows for the optical stimulation and visualization of inhibitory (GABA-releasing) neurons.
Automatically generated - may contain errors
3 protocols using vgat chr2 eyfp
1
Transgenic Mice for Behavioral Studies
2
Transgenic Mice for Optogenetic Neural Imaging
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All procedures were in accordance with the Institutional Animal Care and Use Committee at Nanyang Technological University. Transgenic mice were obtained from the Jackson Laboratory (CaMKII-tTA: 007004; TRE-GCaMP6s: 024742; VGAT-ChR2-EYFP: 014548). Mice were housed in a reversed light cycle (12 h:12 h) in standard cages at around 21 °C and 62% humidity and experiments were generally performed during the dark period. Both male and female hemizygous mice were used. Sample size was determined based on previous studies17 (link),39 (link),53 (link).
3
Optogenetic Manipulation of Neuronal Activity
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All procedures were in accordance with the Institutional Animal Care and Use Committee at Nanyang Technological University (protocol number: A0352). Wild-type (C57BL/6J) and transgenic mice were obtained from Charles River Laboratories and the Jackson Laboratory (CaMKII-tTA: 007004; TRE-GCaMP6s: 024742; VGAT-ChR2-EYFP: 014548), respectively. Mice were housed in a reversed light cycle (12 hours:12 hours) in standard cages, and the experiments were typically performed during the dark period. Both male and female hemizygous mice were used. Sample size was determined on the basis of the standard in the field. Mice were randomly allocated to each group, and no blinding was performed.
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