Particle agglutination kit

Serum concentrations of free T4 (FT4), free T3 (FT3), and thyrotropin (TSH) were measured using ECLIA kit (Roche Diagnostics Ltd., Tokyo, Japan). Normal ranges of serum FT4, FT3, and TSH are 0.9-1.7 ng/dL, 2.3-4.3 pg/mL, and 0.5-5.0 μIU/mL, respectively. TgAb and McAb were measured using a particle agglutination kit (Fujirebio Inc., Tokyo, Japan). A reciprocal titer >1:100 was considered positive. Serum TRAb at onset was measured by radioreceptor assay using a commercial kit (Cosmic Corp., Tokyo, Japan) as part of routine studies. Serum TRAb at the time of sampling was determined by ECLIA kit (third-generation) (Roche Diagnostics Ltd., Tokyo, Japan) using preserved samples. Normal value of TRAb was less than 10% in the radioreceptor assay and 2.0 IU/L in ECLIA. Goiter size in this study was defined as the longitudinal length of the thyroid gland as measured using a vernier caliper.

The serum concentration of free T4 (FT4), free T3 (FT3) and thyrotropin (TSH) were measured using ECLIA (Roche diagnostics Ltd., Tokyo, Japan). The normal range of serum FT4, FT3 and TSH are 0.9-1.7 ng/dL, 2.3-4.3 pg/mL and 0.6-5.0 μIU/mL, respectively. TgAb and McAb were measured using a particle agglutination kit (Fujirebio Inc., Tokyo, Japan). A reciprocal titer of >1:100 was considered positive. Serum TRAb at the onset was measured by a radioreceptor assay using a commercial kit (Cosmic Corporation, Tokyo, Japan) as a part of routine studies. Serum TRAb at sampling was determined using ECLIA (third-generation) (Roche diagnostics Ltd., Tokyo, Japan) with preserved samples. The normal value of TRAb is less than 10% and 2.0 IU/L.