To process the cells or frozen sections of heart tissues, a fixation step was executed using 4% paraformaldehyde for 15 min. Subsequently, permeabilization was achieved with 0.5% Triton X‐100 for a duration of 2 min, followed by blocking with 5% BSA. Incubation of the sections with the primary antibody TOMM20 (1:100 dilution; Proteintech) was carried out overnight at 4°C. The addition of secondary antibodies was followed by the use of DAPI (G1012‐10ML; Servicebio) as a counterstain for the nucleus. The images were captured with the assistance of a fluorescence microscope (Olympus).
Dapi g1012 10ml
Manufactured by Wuhan Servicebio Technology
DAPI (G1012‐10ML) is a fluorescent dye commonly used for nuclear staining in fluorescence microscopy. It binds strongly to adenine-thymine (A-T) rich regions of DNA, allowing for the visualization of cell nuclei. The product is available in a 10 mL volume.
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Lab products found in correlation
2 protocols using dapi g1012 10ml
1
Immunohistochemical Analysis of Cardiac Tissues
2
Analyzing Cell Death in Mouse Hearts
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Transferase‐mediated dUTP nick‐end labeling (TUNEL) staining of frozen mouse heart sections was performed using a cell death detection kit (Millipore) according to the instructions provided by the manufacturer. DAPI (G1012‐10ML; Servicebio) was used to stain the nuclei.
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