Portions (1 mL) of purified magnetosomes suspended in 0.2 μm filtered PBS (prepared as described in section 2.2) were centrifuged at 16,873 g av for 3 min in the FA-45-18-11 fixed angle (45º) rotor of an Eppendorf model 5418 centrifuge (Eppendorf AG, Hamburg, Germany). The pellets were resuspended in 1 mL of 2.5% (v/v) glutaraldehyde in 0.1 M potassium phosphate buffer, pH 7.2, and mixed at 4 rpm for 1 h at room temperature on a TAAB R052 rotator (TAAB Laboratories Equipment Ltd, Aldermaston, Berks, UK). The glutaraldehyde-fixed magnetosome samples were exhaustively dehydrated by a series of washing steps of increasing ethanol concentration (from 50-100% v/v). Sedimented magnetosomes from the last dehydration step were embedded in resin by infiltration of the pellet with a solution containing 50% (v/v) Mollenhauer [41] resin in propylene oxide (Agar Scientific, Stansted, Essex, UK) on a TAAB rotator operated at 4 rpm for 12 h in a fume cupboard, followed by curing in undiluted Mollenhauer resin at 60 °C for another 48 h. Thin sections (120 nm) were cut from the resin block using diamond knives on a Reichert-Jung
Model 5418 centrifuge
Manufactured by Eppendorf
Sourced in Germany
The Eppendorf model 5418 centrifuge is a compact, benchtop centrifuge designed for general laboratory use. It features a maximum speed of 15,000 rpm and a maximum relative centrifugal force (RCF) of 17,000 x g. The centrifuge has a rotor capacity of 18 x 1.5/2.0 mL microtubes.
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Lab products found in correlation
2 protocols using model 5418 centrifuge
1
Magnetosome Purification and Embedding
2
Magnetosome Purification and Embedding
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Portions (1 mL) of purified magnetosomes suspended in 0.2 μm filtered PBS (prepared as described in section 2.2) were centrifuged at 16,873 g av for 3 min in the FA-45-18-11 fixed angle (45º) rotor of an Eppendorf model 5418 centrifuge (Eppendorf AG, Hamburg, Germany). The pellets were resuspended in 1 mL of 2.5% (v/v) glutaraldehyde in 0.1 M potassium phosphate buffer, pH 7.2, and mixed at 4 rpm for 1 h at room temperature on a TAAB R052 rotator (TAAB Laboratories Equipment Ltd, Aldermaston, Berks, UK). The glutaraldehyde-fixed magnetosome samples were exhaustively dehydrated by a series of washing steps of increasing ethanol concentration (from 50-100% v/v). Sedimented magnetosomes from the last dehydration step were embedded in resin by infiltration of the pellet with a solution containing 50% (v/v) Mollenhauer [41] resin in propylene oxide (Agar Scientific, Stansted, Essex, UK) on a TAAB rotator operated at 4 rpm for 12 h in a fume cupboard, followed by curing in undiluted Mollenhauer resin at 60 °C for another 48 h. Thin sections (120 nm) were cut from the resin block using diamond knives on a Reichert-Jung
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