Primary antibodies used: mouse α-tubulin (cat# T5168, RRID:AB_477579; Sigma-Aldrich), rabbit calnexin (cat# ADI-SPA-860-D, RRID:AB_2038898; Enzo Life Sciences), rabbit cathepsin D (cat# ab75852, RRID:AB_1523267; Abcam), rabbit CGAS (cat# 66546, RRID:AB_2799712; Cell Signaling Technology), mouse DNA (cat# CBL186, RRID:AB_9336; Millipore), rabbit EEA1 (cat#, PA1-063A, RRID:AB_2096819; Thermo Fisher Scientific), mouse GAPDH (cat# ADI-CSA-335, RRID:AB_10617247; Enzo Life Sciences), mouse GM130 (cat# 610822, RRID:AB_398141; BD Biosciences), rabbit HSP60 (cat# 12165, RRID:AB_2636980; Cell Signaling Technology), rabbit IRF3 (cat# 4302, RRID:AB_1904036; Cell Signaling Technology), mouse LAMP1 (cat# h4a3, RRID:AB_2296838; DSHB), rabbit Phospho-IRF3 (S396; cat# 4947, RRID:AB_823547; Cell Signaling Technology), rabbit Phospho-STING (S366; cat# 19781, RRID:AB_2737062; Cell Signaling Technology), rabbit Phospho-STING (S365; cat# 72971, RRID:AB_2799831; Cell Signaling Technology), rabbit Phospho-TBK1 (S172; cat# 14590, RRID:AB_2798527; Cell Signaling Technology), rabbit Rab7 (cat# 9367, RRID:AB_1904103; Cell Signaling Technology), rabbit STING (cat# 80231, RRID:AB_2799947; Cell Signaling Technology), rabbit TFEB (cat# 4240, RRID:AB_11220225; Cell Signaling Technology), rabbit TBK1 (cat# 3504, RRID:AB_2255663; Cell Signaling Technology), and rabbit VPS13C (custom, Proteintech).
Hancock-Cerutti W., Wu Z., Xu P., Yadavalli N., Leonzino M., Tharkeshwar A.K., Ferguson S.M., Shadel G.S, & De Camilli P. (2022). ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. The Journal of Cell Biology, 221(7), e202106046.
+ Open protocol