Dapi d3571

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

DAPI (D3571) is a fluorescent dye used for nucleic acid staining. It binds strongly to the adenine-thymine (A-T) rich regions of DNA. DAPI provides a simple and effective method for detecting and visualizing DNA in various biological samples.

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Lab products found in correlation

Pka 9621, by Cell Signaling Technology (1 mentions) Anti ucp1 ab23841, by Abcam (1 mentions) Bodipy 493 503 d3922, by Thermo Fisher Scientific (1 mentions) Isoproterenol i6504, by Merck Group (1 mentions) Glyoxal solution, by Merck Group (1 mentions) Goat anti mouse hrp, by Jackson ImmunoResearch (1 mentions) Rabbit anti ve cadherin, by Cell Signaling Technology (1 mentions) Iba1 antibody, by Fujifilm (1 mentions) Mhcii 1 a 1 e, by BioLegend (1 mentions) Apc conjugated cd11b, by Thermo Fisher Scientific (1 mentions) Anti mmp 13, by Thermo Fisher Scientific (1 mentions)

4 protocols using dapi d3571

Antibodies and Reagents for Adipocyte Research

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Antibodies against COX-2 (12282), PPARγ (2443), PGC1α (2178), and PKA (9621) were from Cell Signaling Technology (Danvers, MA, USA). Polyclonal antibodies to adiponectin and anti-tubulin were kindly provided by Drs. Lily Dong and Feng Liu at UT Health San Antonio (San Antonio, TX, USA) as described previously [40 (link)]. The anti-UCP1 (ab23841) and anti-Plin1 (ab61682) were purchased from Abcam (Discovery Drive, Cambridge, CB2 0AX, UK). BODIPY 493/503 (D3922) and DAPI (D3571) were from Thermo Fisher Scientific (Waltham, MA, USA). PGD2 (41598-07-6), PGE2 (14010), PGI2 (69552-46-1), L-161,982 (10011565), TG6-10-1 (23444), SC-51089 (10011561), and Rosiglitazone (71740) were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). KT5720 (K3761) and Isoproterenol (I6504) were from Sigma-Aldrich (St. Louis, MO, USA). H89 (371963) was purchased from Millipore (Burlington, MA, USA).

Wang C., Zhang X., Luo L., Luo Y., Wu D., Spilca D., Le Q., Yang X., Alvarez K., Hines W.C., Yang X.O, & Liu M. (2022). COX-2 Deficiency Promotes White Adipogenesis via PGE2-Mediated Paracrine Mechanism and Exacerbates Diet-Induced Obesity. Cells, 11(11), 1819.

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Immunofluorescence and Western Blot Analysis

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Glyoxal solution (Cat# 50649) was from Sigma-Aldrich. Rabbit anti-VE-Cadherin (#2158) was from Cell Signaling and was diluted 1:1000 for Western blot analyses. Anti-VE-Cadherin antibody was diluted 1:100 for immunofluorescence assay. Horseradish peroxidase (HRP) conjugated goat antirabbit (sc-2054) secondary Ab was from Santa Cruz Biotechnology, while HRP-goat anti-mouse (115-035-003) was from Jackson ImmunoResearch Laboratories. Alexa Fluor 488 donkey anti-rabbit (A21206), Alexa Fluor 555 Phalloidin (A34055) and DAPI (D3571) were from Thermo Fisher Scientific. GAPDH (G-9) monoclonal HRP has been used in western blot 1:5000 Santa Cruz Biotechnology. RAGE Antagonist, FPS-ZM1 -Calbiochem was from Sigma-Aldrich.

Camillo C., Abramov A., Allen P.J., Castillero E., Roberts E., Xue Y., Frasca A., Moreno V., Kurade M., Robinson K., Spiegel D.A., LaPar D.J., Grau J.B., Assoian R.K., Bavaria J.E., Takayama H., & Ferrari G. (2021). RAGE antagonist peptide mitigates AGE-mediated endothelial hyperpermeability and accumulation of glycoxidation products in human ascending aortas and in a murine model of aortic aneurysm.

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Murine Neuroinflammation Immunostaining Protocol

Cited 6 times

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We obtained murine recombinant IL-33 (580506), AF647-conjugated Aβ (clone: 6E10) antibody (803021), APC-conjugated MHC-II (clone: M5/114.15.2) antibody (107614), FITC-conjugated VCAM1 (clone: MVCAM.A) antibody (105706) and MHC-II (I-A/I-E) (clone: M5/114.15.2) antibody (107601) from BioLegend. We obtained ICAM1-neutralizing (clone: YN1/1.7.4) antibody (BE0020)^{75 (link),76 (link)} and VCAM1-neutralizing (clone: M/K-2.7) antibody (BE0027)^{69 (link),77 (link)} from Bio X Cell. We obtained AF488-conjugated CD11b (clone: M1/70) antibody (53-0112-82), APC-conjugated CD11b (clone: M1/70) antibody (17-0112-83) and biotinylated CD11b (clone: M1/70) antibody (13-0112-82) from eBioscience. ApoE-neutralizing (clone: HJ6.3) antibody was a gift from D. Holtzman^{36 (link)}. We obtained DAPI (D3571) from Life Technologies, and mouse ITGB2 recombinant protein (LS-G14036-10) was from LSBio. We obtained mouse ApoE recombinant protein (MBS955382) from MyBioSource as well as CCR7 neutralizing (clone: 4B12) antibody (MAB3477)^{78 (link)} and VCAM1 antibody (BBA5) from R&D Systems. We obtained recombinant mouse CD44 protein (53953-M08H) from Sino Biological, MeX04 (4920) from Tocris Bioscience and Iba1 antibody (019-19741) from Wako.

Lau S.F., Wu W., Wong H.Y., Ouyang L., Qiao Y., Xu J., Lau J.H., Wong C., Jiang Y., Holtzman D.M., Fu A.K, & Ip N.Y. (2023). The VCAM1–ApoE pathway directs microglial chemotaxis and alleviates Alzheimer’s disease pathology. Nature Aging, 3(10), 1219-1236.

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Protein Expression Analysis of Visfatin, NF-κB, and MMP-13

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The primary antibodies used in this study included anti-Visfatin (PA1-1045, Pierce Scientific, Hemel Hempstead, UK), α-NFκB (SC-8008, Santa Cruz Biotech, Santa Cruz, CA, USA), and anti-MMP-13 (11365013, Thermo Scientific, Hemel Hempstead, UK). The secondary antibodies used in this study were Alexa Fluor^® 555 anti-rabbit H + L (A-21428), Alexa Fluor^® 488 anti-mouse IgG1 (A-21121), and DAPI (D3571) (Life Technologies, Warrington, UK).

Philp A.M., Butterworth S., Davis E.T, & Jones S.W. (2021). eNAMPT Is Localised to Areas of Cartilage Damage in Patients with Hip Osteoarthritis and Promotes Cartilage Catabolism and Inflammation. International Journal of Molecular Sciences, 22(13), 6719.

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