Total RNA was reverse transcribed using a PrimeScript RT Reagent Kit (Vazyme, Nanjing, China) and a miRNA qPCR Quantitation Kit (Tiangen, Beijing, China) to synthesize cDNA. Quanti cation of NEAT1 expression was normalized to GAPDH expression, and miR-30a-3p, miR-30a-5p expression was normalized to U6 expression using the 2 -ΔΔCt method. RT-qPCR was performed using the following primers: lncRNA NEAT1 sense, 5'-GAGTGGCTGTTGGAGTCGGTATTG-3' and antisense, 5'-AACTTCCTCCTCCTAAGCCTCTGG-3'; miR-30a-3p sense, 5'-ACACTCCAGC TGGGCTTTCAGTCGGATG-3' and antisense, 5'-CTCAACTGGTGTCGTGGAGTC GGCAATTCAGTTGAGGCTGCAAACATC-3'; miR-30a-5p sense, 5'-ACACTCCA GCTGGGTGTAAACATCCTCGAC-3' and antisense, 5'-CTCAACTGGTGTCGTG GAGTCGGCAATTCAGTTGAGCTTCCAGTCGAGG-3'; U6 sense, 5'-CTCGCTTC GGCAGCACA-3' and antisense, 5'-AACGCTTCACGAATTTGCGT-3'; GAPDH sense, 5'-GGTGGTCTCCTCTGACTTCAACA-3' and antisense, 5'-CCAAATTCGTT GTCATACCAGGAAATG-3'.
Mirna qpcr quantitation kit
Manufactured by Tiangen Biotech
Sourced in China
The MiRNA qPCR Quantitation Kit is a laboratory equipment designed for the quantitative analysis of microRNA (miRNA) expression levels using real-time PCR technology. The kit provides the necessary reagents and protocols to perform accurate and reliable miRNA quantification in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mirna qpcr quantitation kit
1
Quantification of lncRNA NEAT1 and miRNAs
2
Quantification of lncRNA NEAT1 and miRNAs
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Total RNA was reverse transcribed using a PrimeScript RT Reagent Kit (Vazyme, Nanjing, China) and a miRNA qPCR Quantitation Kit (Tiangen, Beijing, China) to synthesize cDNA. Quanti cation of NEAT1 expression was normalized to GAPDH expression, and miR-30a-3p, miR-30a-5p expression was normalized to U6 expression using the 2 -ΔΔCt method. RT-qPCR was performed using the following primers: lncRNA NEAT1 sense, 5'-GAGTGGCTGTTGGAGTCGGTATTG-3' and antisense, 5'-AACTTCCTCCTCCTAAGCCTCTGG-3'; miR-30a-3p sense, 5'-ACACTCCAGC TGGGCTTTCAGTCGGATG-3' and antisense, 5'-CTCAACTGGTGTCGTGGAGTC GGCAATTCAGTTGAGGCTGCAAACATC-3'; miR-30a-5p sense, 5'-ACACTCCA GCTGGGTGTAAACATCCTCGAC-3' and antisense, 5'-CTCAACTGGTGTCGTG GAGTCGGCAATTCAGTTGAGCTTCCAGTCGAGG-3'; U6 sense, 5'-CTCGCTTC GGCAGCACA-3' and antisense, 5'-AACGCTTCACGAATTTGCGT-3'; GAPDH sense, 5'-GGTGGTCTCCTCTGACTTCAACA-3' and antisense, 5'-CCAAATTCGTT GTCATACCAGGAAATG-3'.
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