The largest database of trusted experimental protocols

Pinpoint side rna isolation kit

Manufactured by Zymo Research
Sourced in United States

The Pinpoint Side RNA Isolation Kit is a tool designed to isolate RNA from biological samples. It is a compact and efficient solution for extracting high-quality RNA for various downstream applications.

Automatically generated - may contain errors

2 protocols using pinpoint side rna isolation kit

1

RNA Isolation and Sequencing from Mouse Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA samples were isolated from fresh mouse tissues using RNA-Bee (TEL-TEST, Inc., Friendswood, TX, USA) or from formalin-fixed Paraffin Embedded (FFPE) slides using the Pinpoint Side RNA isolation Kit (Zymo Research, Cat R1007). RNA sequencing libraries were prepared with Kapa RNA HyperPrep Kit with RiboErase (Kapa Biosystems, Cat KR1351). Sequencing runs were performed on Illumina Hiseq 2500 in the single read mode of 51 cycles of read 1 and 7 cycles of index with V4 Kits. Real-time analysis (RTA) 2.2.38 software was used to process the image analysis and base calling. Reverse transcription was performed as described previously 36 (link), and RT-qPCR assays were carried out using SYBR GreenER qPCR Super Mix Universal (11762, Invitrogen) with specific primers (Supplemental Table 3) on the 7500 Real-Time PCR system (Thermo Fisher Scientific).
+ Open protocol
+ Expand
2

RNA Isolation and Sequencing from Mouse Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA samples were isolated from fresh mouse tissues using RNA-Bee (TEL-TEST, Inc., Friendswood, TX, USA) or from formalin-fixed Paraffin Embedded (FFPE) slides using the Pinpoint Side RNA isolation Kit (Zymo Research, Cat R1007). RNA sequencing libraries were prepared with Kapa RNA HyperPrep Kit with RiboErase (Kapa Biosystems, Cat KR1351). Sequencing runs were performed on Illumina Hiseq 2500 in the single read mode of 51 cycles of read 1 and 7 cycles of index with V4 Kits. Real-time analysis (RTA) 2.2.38 software was used to process the image analysis and base calling. Reverse transcription was performed as described previously 36 (link), and RT-qPCR assays were carried out using SYBR GreenER qPCR Super Mix Universal (11762, Invitrogen) with specific primers (Supplemental Table 3) on the 7500 Real-Time PCR system (Thermo Fisher Scientific).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!