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Avidin biotin blocking kit

Manufactured by Zymo Research
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Sourced in Cameroon

The Avidin/Biotin blocking kit is a laboratory tool designed to help researchers manage the potential interference of avidin-biotin interactions in their experiments. It provides a reliable method to block or mask biotin-binding sites, which can be useful in various applications where such interactions need to be controlled or eliminated.

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Lab products found in correlation

Anti goat hrp dab cell and tissue staining kit, by R&D Systems (2 mentions) Goat anti mouse il 15, by R&D Systems (2 mentions)

2 protocols using avidin biotin blocking kit

1

Visualizing IL-15 Expression in Tumor Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors from TS/A cells infected with Ad.mIL-15, Ad.mIL-15 and Ad.mIL-15Rα or Ad.null each at an MOI of 100 were resected, fixed in OCT (SAKURA-Finetek, Torrance, CA) and sectioned by cryostat. Tissue sections were treated with 0.3% hydrogen peroxide, then treated with Avidin/Biotin blocking kit (Zymed, South San Francisco, CA) in accordance with the manufacturer’s instructions. The sections were stained with goat anti-mouse IL-15 (R &D systems). The slides were washed and stained using the anti-goat HRP-DAB Cell and Tissue staining kit (R&D Systems) as per the manufacturer’s instructions and counterstained with hematoxylin.
Morris J.C., Ramlogan-Steel C.A., Yu P., Black B.A., Mannan P., Allison J.P., Waldmann T.A, & Steel J.C. (2014). Vaccination with Tumor Cells Expressing IL-15 and IL-15Rα Inhibit Murine Breast and Prostate Cancer. Gene therapy, 21(4), 393-401.
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2

Visualizing IL-15 Expression in Tumor Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors from TS/A cells infected with Ad.mIL-15, Ad.mIL-15 and Ad.mIL-15Rα or Ad.null each at an MOI of 100 were resected, fixed in OCT (SAKURA-Finetek, Torrance, CA) and sectioned by cryostat. Tissue sections were treated with 0.3% hydrogen peroxide, then treated with Avidin/Biotin blocking kit (Zymed, South San Francisco, CA) in accordance with the manufacturer’s instructions. The sections were stained with goat anti-mouse IL-15 (R &D systems). The slides were washed and stained using the anti-goat HRP-DAB Cell and Tissue staining kit (R&D Systems) as per the manufacturer’s instructions and counterstained with hematoxylin.
Morris J.C., Ramlogan-Steel C.A., Yu P., Black B.A., Mannan P., Allison J.P., Waldmann T.A, & Steel J.C. (2014). Vaccination with Tumor Cells Expressing IL-15 and IL-15Rα Inhibit Murine Breast and Prostate Cancer. Gene therapy, 21(4), 393-401.
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