S 220 ultrasonic processor

From 2006 to 2014, we sampled figs containing mature fig wasps across Southeast Asia. See Figure 1A is the map of sampling locations and Table 1 provides details. Three locations were included. One is from Hainan Island (DA) and two are from the continent (VH and SCBG) . The distances between each two sampling locations are 822 km (SCBG-VH), 498 km (SCBG-DA), and 532 km (VH-DA). In each location, figs were collected from trees 3-5 m away from each other. We chose 10-30 figs from each site and sealed them individually in fine-mesh bags under ambient temperature until the mature wasps emerge. The sp1 wasps were identified and collected, then preserved in 95% ethanol under -20 • C until DNA extraction. The DNA pool of each sample were created for all mature female wasps in a single fig by mixing extraction of genomic DNA. We finally obtained 26 samples for DNA extraction. Genomic DNA was extracted following the method outlined in a previous study (Tian et al., 2015) (link). The genomic DNA was fragmented with the S220 Ultrasonic Processor (Covaris, United States). After end repairing and phosphorylating, common adapters were ligated to the fragments for denaturing and amplifying. The prepared libraries were sequenced with the Illumina HiSeq2500 (Illumina,