Potassium dihydrogen phosphate

Ritonavir (Polymorphic Form II) was obtained from Anhui Biochem United Pharmaceutical Co., Ltd. (Hefei, China) that was used without further purification. Ritonavir Reference Standard (99.3%) was purchased from The United States Pharmacopeial Convention (North Bethesda, MD, USA). PVPVA (Kollidon^® VA 64) was supplied by BASF Corporation (Ludwigshafen, Germany). Sorbitan monolaurate (Span 20-LQ-(AP)) was obtained from Croda Inc. (East Yorkshire, UK). The ultrapure water was purified by the Milli-Q^® Advantage A10^® water purification system (Millipore, MA, USA). Acetonitrile of high performance liquid chromatography (HPLC) grade was obtained by Thermo Fisher Scientific (Newington, NH, USA). Methanol was provided by Adamas Pharmaceuticals, Inc. (Shanghai, China). Potassium dihydrogen phosphate and phosphoric acid were supplied from Tianjin Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China).

SM was obtained from Shanxi Taihang Pharmaceutical Co., Ltd. (Shanxi, China). Atorvastatin calcium salt trihydrate was supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 12 hydration disodium hydrogen phosphate, potassium chloride, sodium chloride, potassium dihydrogen phosphate, and sodium dihydrogen phosphate were all purchased from Tianjin Zhiyuan Chemical Reagent Co., Ltd. (Tianjin China). The liver tissues of each mice were fixed in 4% paraformaldehyde at 4°C for 24 h, then the fixed tissues were embedded in paraffin and cut into 3-μm sections with a microtome (Leica, Nussloch, Germany). paraformaldehyde (analytical grade) was supplied by Damao Chemical Reagent Factory (Tianjin). Hematoxylin–eosin (H&E) and neutral gum were purchased from Beijing Legend Biotech Co., Ltd. (Beijing, China) and Shanghai Yiyang Instrument Co., Ltd. (Shanghai, China), respectively. Triglyceride, total cholesterol, glucose, LDL-C, and HDL-C were measured by commercially available kits obtained from Shanghai Rongsheng Biotech Co., Ltd., while insulin was detected using kits supplied by Wuhan Huamei Biological Engineering Co. Ltd. (Wuhan, China).